We narrowed to 22,836 results for: Sis

1. sh-Myocardin

   Plasmid

   #100768

   Purpose

   expression of shRNA targeting MYOCD

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

   Insert

   sh-Myocardin
   

   Use

   RNAi

   Promoter

   H1

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_PACSIN3-1/1

   Plasmid

   #91335

   Purpose

   Protein expression and purification of human SH3 domain construct PACSIN3-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   PACSIN3-1/1 (PACSIN3 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RAB14 3'UTR D1

   Plasmid

   #61490

   Purpose

   luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   RAB14 3' UTR (RAB14 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   contains position 837-1637 (numbering relative to…

   Promoter

   PGK

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pOT2 3GLA

   Plasmid

   #71764

   Purpose

   gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

   Depositor

   Matthias Soller

   Article

   Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMath3-luc

   Plasmid

   #51312

   Purpose

   Luciferase reporter driven by the Math3 promoter

   Depositor

   Ryoichiro Kageyama

   Article

   Tsuda et al J Biol Chem. 1998 Mar 13;273(11):6327-33

   Insert

   Math3 promoter (-2.8k- +196) (Neurod4 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pEG302 5aa SunTag VP64 gRNA17 (FWA)

   Plasmid

   #115482

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF1-ERBB4 (E563K)

   Plasmid

   #29541

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

   Plasmid

   #52540

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Expression

   Mammalian

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe hygro NFS1 CD

   Plasmid

   #102978

   Purpose

   Expression of NFS1 C381A mutant

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   NFS1 C381A (NFS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   C381A catalytic site mutant, Silent mutations to …

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pDESTFLAGHApuro3-Ago2Q545AK570A

    Plasmid

    #14105

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Gln545 to Ala and Lys570 to Ala

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pMf3tvtaulacZ

    Plasmid

    #37264

    Depositor

    Patricia Labosky

    Article

    Kloetzli et al Genesis. 2001 Feb;29(2):60-71.

    Insert

    Foxb1 (Foxb1 Mouse)
    

    Tags

    taulacZ

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3 Flag BimL (S44D)

    Plasmid

    #24235

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44D) (BCL2L11 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed serine 44 to aspartic acid

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pIS47 C20orf139 3'UTR mut

    Plasmid

    #14490

    Depositor

    David Bartel

    Article

    Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

    Insert

    C20orf139 3'UTR mutant (SRXN1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CATTCC’s were changed to AAGTAC's

    Available Since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL2-MCT2Prom4.2

    Plasmid

    #87347

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 4.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    4.2 kbp proximal promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24a_UDP_Xase_E113

    Plasmid

    #73159

    Purpose

    Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113A

    Depositor

    Sandra Gabelli

    Article

    Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

    Insert

    pET24a
    

    Expression

    Bacterial

    Mutation

    change glutamate 113 to alanine

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-HA-REDD1 C140S (#622)

    Plasmid

    #99947

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C140S

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET151-Vta1(280-330) S278C

    Plasmid

    #87739

    Purpose

    Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-277, mutated Ser 278 to Cys

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG/MKRN1(110-482)

    Plasmid

    #78754

    Purpose

    To overexpress MKRN1(110-482) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    110-482

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNUT N6His mICA NG

    Plasmid

    #70162

    Purpose

    Expresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydrase

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Wang et al Biochem J. 2007 Aug 15;406(1):85-95.

    Insert

    mouse inhibitor of carbonic anhydrase
    

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn470Asp and Asn645Asp

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5A7-T

    Plasmid

    #92020

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. p5A3-T

    Plasmid

    #92019

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET151-Vps4(81-437)

    Plasmid

    #87734

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-80

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBGO

    Plasmid

    #62671

    Purpose

    Donor vector for mFTH1 knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FTH1
    

    Use

    Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-HA-RIPK3 V460P

    Plasmid

    #78814

    Purpose

    To overexpress RIPK3 V460P in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V460P

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGB1-mCRT(P-domain)

    Plasmid

    #83519

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    P domain (contains residues 187-283)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pGB1-mCRT(C-domain)

    Plasmid

    #83518

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    C domain (contains residues 340-399)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pCDNA3 Flag BimL (S44A)

    Plasmid

    #24234

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    BimL (S44A) (BCL2L11 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Serine 44 to Alanine

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pFRT-TODestFLAGHA_RBPMS2_HindIII

    Plasmid

    #65666

    Purpose

    FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDESTFLAGHApuro3-Ago2K570A

    Plasmid

    #14102

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Lys570 to Ala

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

30. JW630

    Plasmid

    #64807

    Purpose

    TCP4 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    TCP4 (TCP4 Mustard Weed)
    

    Tags

    GAL4 Activation domain and HA

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pICSL11061

    Plasmid

    #68255

    Purpose

    Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter_AtU6-26 + sgRNA_BolC.GA4a-1
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3 T7 BimL (T56D)

    Plasmid

    #24532

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bim L(T56D) (BCL2L11 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Changed threonine 56 to aspartic acid

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

33. pET151-hUCH37 (1-228)

    Plasmid

    #61932

    Purpose

    Expression of hUCH37 (1-228) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (1-228) (UCHL5 Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Stop codon introduced after residue K228

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

34. RAB14 3'UTR D2

    Plasmid

    #61491

    Purpose

    luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    RAB14 3' UTR (RAB14 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains position 837-1637 (numbering relative to…

    Promoter

    PGK

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pAc5.1A-Dm-miR-13b-1

    Plasmid

    #22762

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

    Insert

    micro RNA 13b-1 (mir-13b-1 primary transcript, Fly)
    

    Use

    Drosophila expression

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52542

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEYFP-N1-hRIP3-FL G457D

    Plasmid

    #61367

    Purpose

    Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with G457D mutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed G457 to D

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pEYFP-N1-hRIP3-FL N464D

    Plasmid

    #61369

    Purpose

    Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with N464D mutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed N464to D

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pFA6a-GFP (F64L, S65T, V163A)-His3MX6

    Plasmid

    #53185

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    HIs3MX6 (HIS3 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pEG610

    Plasmid

    #40195

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    contains aa 1-355; exon 2 recoded to be RNAi-resi…

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only