We narrowed to 40,900 results for: Eras
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Plasmid#188256PurposePlasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only
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pNeuLite PEA3 mt
Plasmid#16248DepositorInsertHer-2/neu promoter (ERBB2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPEA3 mt: PEA3 motif AGGAAG changed to AGCTCGAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pZS1[TreR-T,LacI-T]
Plasmid#60770PurposeContains PI driving expression TreR-T, and PI driving expression of LacI-T.DepositorInsertsTreR-T
Dimeric LacI with the TAN DBD
UseSynthetic BiologyExpressionBacterialMutationDimeric LacI with the TAN DBD. and LacI/GalR repr…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_01
Plasmid#158102Purpose5'UTR stem-loop luciferase reporter: 0kcal/molDepositorInsert5'UTR synthetic stem-loop 0kcal/mol
UseLuciferaseExpressionMammalianPromoterPGKAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-CIBN
Plasmid#183937PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposureDepositorInserttdPCP-CIBN (CIB1 Mustard Weed, Synthetic)
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87E
Plasmid#36059DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-TbID-emerin
Plasmid#175099PurposeLentiviral expression of V5-TbID-tagged mouse EmdDepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
K15-TK/pGL3
Plasmid#44267DepositorInsertK15 promoter (-4.8) (Krt15 Mouse)
UseMouse Targeting; Thymidine kinaseTagsHSV-1 TKExpressionMammalianMutationContains the murine K15 promoter (-4.8) fragmentPromotermurine K15 promoter (-4.8) fragmentAvailable SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 2-no linker
Plasmid#176921Purposeexpress Granulin 2 without linker 3 in mammalian cellsDepositorInsertGranulin 2 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 1+linker2
Plasmid#176918Purposeexpress Granulin 1 with linker 2 in mammalian cellsDepositorInsertGranulin 1+linker2 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-LPPRC-mod
Plasmid#48168Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame elementDepositorInsertLRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
UseLuciferaseMutationA/T mutation in the ATG of the uORFAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
TagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…Available SinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-RL-BAD
Plasmid#78859PurposeDULIP positive control (bait) for assay establishmentDepositorInsertBCL2 associated agonist of cell death (BAD Human)
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
1068 pGL3 FasL promoter (no SV40 prom)
Plasmid#9029DepositorInsertFas ligand promoter (FASLG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationRemoved the SV40 promoter from pGL3Available SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet1 enhancer E
Plasmid#63880PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 enhancer fragment E
UseLuciferaseExpressionMammalianPromoterSV40Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[TreR-T]
Plasmid#60754PurposeContains PI driving expression TreR-T, the Trehalose inducible chimera with the TAN DBD.DepositorInsertsTreR-T
GalS-T
UseSynthetic BiologyExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS54 ADAR 3'UTR mut
Plasmid#14488DepositorInsertADAR 3'UTR mutant (ADAR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_1
Plasmid#155059PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX1 Chromo
Plasmid#59697PurposeContains histone modification interacting domain (CBX1 Chromo)DepositorAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4+linker5
Plasmid#176924Purposeexpress Granulin 4 with linker 5 in mammalian cellsDepositorInsertGranulin 4+linker5 (GRN Human)
TagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_PDPR_exon_deletion_1
Plasmid#155065PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A
Plasmid#36058DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJWK_VD_02
Plasmid#158103Purpose5'UTR stem-loop luciferase reporter: -30kcal/molDepositorInsert5'UTR synthetic stem-loop -30kcal/mol
UseLuciferaseExpressionMammalianPromoterPGKAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_190-303
Plasmid#118822PurposeBacterial expression of hnRNPA2 truncation (residues 190 to 302).DepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis tag and TEVExpressionBacterialMutationcontains amino acids 190-302Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi
Plasmid#89740PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-GCN4-dCEN-INCENP
Plasmid#108510Purposeexpression of vsv-GCN4-dCEN-INCENPDepositorInsertGCN4-dCEN-INCENP (INCENP Human)
TagsVSVExpressionMammalianMutationreplace CEN box for GCN4 dimerazation domainPromoterCMVAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4-GFP
Plasmid#67392PurposeMammalian expression of hArf4 with C-term GFP tagDepositorInsertARF4 (ARF4 Human)
TagsGFPExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_Ptbp_ex8_3
Plasmid#155083PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKTop::tse5-CT (1169-1281)
Plasmid#192955PurposeFor membrane insertion study; Expresses tse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInserttse5-CT (1169-1281)
ExpressionBacterialMutationencodes for tse5 (1169-1281)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_SFRS7_exon_deletion_3
Plasmid#155071PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only