We narrowed to 30,619 results for: ica

1. pUCmini-UGCG-tdTomato

   Plasmid

   #225879

   Purpose

   Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

   Depositor

   Yohei Sato

   Article

   Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG1280 NLS-mCherry-FIREtag-NES

   Plasmid

   #205172

   Purpose

   expresses NLS-mCherry-FIREtag-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   NLS-mCherry-FIREtag-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ki67 (LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183743

   Purpose

   Expresses Ki67 (LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   LR domain of Ki67

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-Cx35-Halo-C-end

   Plasmid

   #71512

   Purpose

   Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

   Depositor

   John O'Brien

   Article

   Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

   Insert

   Connexin 35
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPSA5Cas9syn2

   Plasmid

   #206963

   Purpose

   S. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGAT

   Depositor

   Daijie Chen

   Article

   Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRAIP-SFB

   Plasmid

   #101769

   Purpose

   Mammalian expression of TRAIP fusion protein

   Depositor

   Michael Huen

   Article

   Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

   Insert

   TRAIP / RNF206 (TRAIP Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SHC003BSD-DelGFP

   Plasmid

   #133301

   Purpose

   EGFP reporter was removed and the selection marker was modified as blasticidin in the construct of SHC003. This construct could be used for expression of a gene by lentiviral approach.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET23a-HsRPS27a

   Plasmid

   #69561

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. p2H12

   Plasmid

   #221142

   Purpose

   cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDAS12124_PEAR-GFP-preedited

    Plasmid

    #177179

    Purpose

    PEAR-GFP control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPSA5Cas9syn

    Plasmid

    #206965

    Purpose

    S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

    Depositor

    Daijie Chen

    Article

    Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHLP710

    Plasmid

    #203740

    Purpose

    Integration of OsTIR1-3xMyc at NEUT5L locus

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    OsTIR1
    

    Use

    Candida albicans integrating vector

    Tags

    3xMyc

    Expression

    Mutation

    Promoter

    ACT1 (C. albicans)

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. XMRV vp62

    Plasmid

    #26189

    Depositor

    Joseph DeRisi

    Article

    Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

    Insert

    XMRV VP62 (gag Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

14. RIP-Timer (DM#285)

    Plasmid

    #15109

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    insulin II promoter (Ins2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSC12

    Plasmid

    #104786

    Purpose

    pNB184-Cas9 entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB-CMV-MCS-Puro GEM-LTB4mut

    Plasmid

    #202642

    Purpose

    Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    GEM-LTB4mut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N1-mt-ro1GFP

    Plasmid

    #82407

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/T65S/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTK82_GFP-4.1G

    Plasmid

    #46353

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    4.1G (EPB41L2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pBABE

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-cMSPdH5

    Plasmid

    #199771

    Purpose

    Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH5
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    external His-Tags flanking the C and N termini

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-miniSOG-C1

    Plasmid

    #54801

    Purpose

    Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SpyCatcher002-oHept

    Plasmid

    #124671

    Purpose

    Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHept
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

    Plasmid

    #20946

    Purpose

    3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    halorhodopsin
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pH6HTC_IGF2BP3-Halo

    Plasmid

    #175310

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    IGF2BP3 (IGF2BP3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSC10

    Plasmid

    #104784

    Purpose

    pAH595-gRNA 2xplex entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-memGFP-P2A

    Plasmid

    #80809

    Purpose

    Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    pME-memGFP-P2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pR-mClover3

    Plasmid

    #228605

    Purpose

    Mammalian expression of mClover3, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBTK1006 (pSL5)

    Plasmid

    #191001

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTP924 pHAGE2 CMV GFP

    Plasmid

    #199392

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHiUGE GS-gRNA (hSyn-Cre) backbone

    Plasmid

    #200368

    Purpose

    Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGBW-m4046979

    Plasmid

    #145611

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pPROEX Hsp104 (1-575)

    Plasmid

    #1318

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 (1-575) (HSP104 Budding Yeast)
    

    Use

    Tags

    6X His + TEV cleavage

    Expression

    Bacterial

    Mutation

    aa1-575 of Hsp104

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHiUGE HA-epitope donor ORF0

    Plasmid

    #200371

    Purpose

    Tagging endogenously expressed proteins with HA epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    HA epitope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SGL40C.EFS.dTomato

    Plasmid

    #89395

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. OSCA3.1

    Plasmid

    #136596

    Purpose

    Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA3.1 (ERD4 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTE1062

    Plasmid

    #172356

    Purpose

    Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

    Inserts

    scbR (SCO6265 )
    kasOp
    

    Use

    Synthetic Biology; Integrative into streptomyces …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge