We narrowed to 23,940 results for: nes

1. pCMV5 Flag-Smad1 (4SP/AP)

   Plasmid

   #14043

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

   Insert

   Smad1 (4SP/AP) (SMAD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Four Serine to Alanine mutations in the linker re…

   Promoter

   Available since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

2. GFP-mROCK1-delta4

   Plasmid

   #101292

   Purpose

   Mammalian expression of Rock1-GFP for the study of localisation signaling.

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   Rock1 (Rock1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   76-1354 aa

   Promoter

   CMV

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. hME-pOX

   Plasmid

   #178181

   Purpose

   Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

   Depositor

   Ming-Feng Tsai

   Article

   Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

   Insert

   human MCU-EMRE fusion protein
   

   Use

   Tags

   1D4

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBabe-vHras-zeo

   Plasmid

   #17756

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   vHras (genomic0 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V12D

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pLJC6-NADK-3xFLAG

   Plasmid

   #163452

   Purpose

   lentiviral overexpression vector for human NADK

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   NADK (NADK Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available since

   March 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAtCas9_1

   Plasmid

   #91156

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH6HTC_YTHDF1-Halo

   Plasmid

   #175301

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   YTHDF1 (YTHDF1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWZL-Stat5a1*6

   Plasmid

   #130669

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7, lacO

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBS-Foxd3 in situ

    Plasmid

    #37098

    Depositor

    Patricia Labosky

    Article

    Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

12. Lenti-sgRbm10/Cre

    Plasmid

    #89648

    Purpose

    Expresses an Rbm10-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rbm10 (Rbm10 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Dlx6

    Plasmid

    #15539

    Depositor

    John Rubenstein

    Article

    Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

    Insert

    Dlx6 (Dlx6 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pMOD_A0503

    Plasmid

    #91013

    Purpose

    Module A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-mROCK1-delta6

    Plasmid

    #101294

    Purpose

    Mammalian expression of Rock1-GFP for the study of localisation signaling.

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (Rock1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    375-1354 aa

    Promoter

    CMV

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JEH127

    Plasmid

    #179299

    Purpose

    Expresses dCas9 fused to VPR

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    CAG

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmGW182

    Plasmid

    #22419

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ2259 - Intron GG2 - 900 bp

    Plasmid

    #159881

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCol2 flu CIITA (P#294)

    Plasmid

    #14651

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

    Insert

    CIITA (CIITA Human)
    

    Use

    Tags

    flu

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pCMB-TGN61r

    Plasmid

    #61175

    Purpose

    Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtSYP61
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. XE7 Xwnt-8

    Plasmid

    #16869

    Depositor

    Randall Moon

    Article

    Christian et al Development. 1991 Apr. 111(4):1045-55.

    Insert

    Xwnt-8 RNA (wnt8a.L Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pNRB143

    Plasmid

    #36452

    Depositor

    Douglas Bishop

    Article

    Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

    Insert

    RAD54 (RAD54 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pMOD_C3013

    Plasmid

    #91100

    Purpose

    Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    PvUbi2

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Halo-WIPI2B R108E

    Plasmid

    #176004

    Purpose

    Expresses WIPI2B (R108E) fused to Halo in mammalian cells

    Depositor

    Erika Holzbaur

    Article

    Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

    Insert

    WIPI2B (R108E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Arginine 108 to Glutamate

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHD-mW-attPCC-FRT (pTL717)

    Plasmid

    #115158

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    mW attP-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. iRFP713-FRB-PIP5K1A

    Plasmid

    #202743

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-sgSmad4/Cre

    Plasmid

    #89651

    Purpose

    Expresses a Smad4-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Smad4 (Smad4 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKD282

    Plasmid

    #125072

    Purpose

    Expression of SO_4427 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4427 (SO_4427 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pALPS_3'LTR GFP@gag start SFFV-

    Plasmid

    #101337

    Purpose

    Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. iRFP713-FRB-Mss4-Kina

    Plasmid

    #202744

    Purpose

    expresses recruitable fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. V1-MESA-45F-M-dCas9

    Plasmid

    #84504

    Purpose

    MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS/KS-mId2sp

    Plasmid

    #16044

    Depositor

    Robert Benezra

    Article

    Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

    Insert

    Id2 (Id2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cDNA nucleotides 1-195

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pTK-EdnrbE2-Citrine

    Plasmid

    #127770

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-lambdaN-HA-DmStaufen_B

    Plasmid

    #145947

    Purpose

    Insect Expression of DmStaufen

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    DmStaufen (stau Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PH-PLCd1-mNGx3

    Plasmid

    #202753

    Purpose

    expresses fluorescent protein-tagged lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSR26-2

    Plasmid

    #104335

    Purpose

    Expresses ompR from a TetR reuglated promoter under inducible ATC control.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTetO1

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

    Plasmid

    #159886

    Purpose

    Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [4-1] - Promoter - Psmu-1(no_atg)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

    Plasmid

    #159887

    Purpose

    Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [4-1] - Promoter - Psmu-2 (no_start)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge