159,481 results

1. pEMS1937

   Plasmid

   #79782

   Purpose

   High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   Ple260
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Expression

   Mutation

   Promoter

   Ple260 (PAX6 MiniPromoter)

   Available since

   Sept. 14, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSP2-48-merTetO-EFS-BLaR

   Plasmid

   #118712

   Purpose

   Contains an optimized 48-mer TetO repeat for imaging of desired loci

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Inserts

   Blasticidin resistance gene
   EFS-NS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

   Plasmid

   #128424

   Purpose

   Human Codon Optimized dCas9-DNMT3A3L

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-pur-CAG-mCherry

   Plasmid

   #159458

   Purpose

   AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Use

   Tags

   Palmitoylation signal*

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7MEQ

   Plasmid

   #176412

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   8 x His Tag and Honeybee melittin signal

   Expression

   Mutation

   etmprss2_S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV-PE6e

   Plasmid

   #207855

   Purpose

   Mammalian expression of PE6e prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6e
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Cas9(H840A, K918A, K775R)

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT-ChemoG-ATP

   Plasmid

   #193818

   Purpose

   CMV overexpression of ChemoG-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

    Plasmid

    #210725

    Purpose

    NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    C2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP288

    Plasmid

    #113460

    Purpose

    human Flag tagged Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRCherry2.1

    Plasmid

    #108099

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-FRB-LgBiT

    Plasmid

    #182650

    Purpose

    FRB-LgBiT

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    FRB-LgBiT fusion
    

    Use

    Tags

    LgBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dyn2-pmCherryN1

    Plasmid

    #27689

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Dynamin 2 (DNM2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

    Plasmid

    #113160

    Purpose

    A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. c-myc promoter (Del 1)

    Plasmid

    #16601

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 1) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-1 (XmnI->PvuII)

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. CD44S pWZL-Blast

    Plasmid

    #19126

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    CD44 standard (CD44 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDG1-PHP.eB

    Plasmid

    #220799

    Purpose

    AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    E4, E2A, VA, AAV2 rep and PHP.eB cap genes
    

    Use

    AAV; Aav helper vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. sfGFP-pBAD

    Plasmid

    #54519

    Purpose

    Localization: Protein Expression Vector , Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and SfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pLentiCRISPRv2 sgIRF3#5

    Plasmid

    #127642

    Purpose

    Knock-out of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 sgRNA (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMTCbLCVA::CH42

    Plasmid

    #22786

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    A DNA containing CHLI fragment
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGWB506

    Plasmid

    #74848

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pYIC

    Plasmid

    #18673

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Type

    Empty backbone

    Use

    Tags

    3Myc-EYFP-HA-His6 and ECFP-HA-His6

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAA3.stelN

    Plasmid

    #112011

    Purpose

    Expresses TelN enzyme

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Insert

    TelN
    

    Use

    Tags

    strep and his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLENTI_hACE2_PURO

    Plasmid

    #155295

    Purpose

    Lentiviral vector to generate hACE2 stable expressing cell line

    Depositor

    Raffaele De Francesco

    Insert

    hACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    C9

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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