165,947 results

1. pCW57.1 N-term GFP tTA

   Plasmid

   #107551

   Purpose

   All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP (optional)

   Expression

   Mammalian

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-3xFLAG-TurboID-MCS

   Plasmid

   #186813

   Purpose

   CAG promoter-driven expression of TurboID-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Tags

   3xFLAG-TurboID

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGFP-Rab8A

   Plasmid

   #24898

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH41822

   Plasmid

   #48021

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #125692

   Purpose

   Global labeling of inhibitory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

   Plasmid

   #48322

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and Thioredoxin

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 TEV (full-length)

   Plasmid

   #64276

   Purpose

   Expresses tobacco etch virus protease (TEV)

   Depositor

   Xiaokun Shu

   Article

   To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Insert

   Tobacco etch virus (TEV) protease
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX311-Cas9

    Plasmid

    #118018

    Purpose

    Constitutive expression of Cas9

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Cas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-Bla

    Plasmid

    #60526

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nrf1-Myc pcDNA3.1(+)-C-Myc

    Plasmid

    #169125

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJ1021

    Plasmid

    #228761

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 gRNAs (Ift88 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SeeSaw Reporter (SSR2.0)

    Plasmid

    #50393

    Purpose

    SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

    Depositor

    Pablo Huertas Sánchez

    Article

    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

    Insert

    SSR2.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM1573

    Plasmid

    #40239

    Depositor

    Susan Golden

    Article

    Andersson et al Methods Enzymol. 2000;305:527-42.

    Insert

    Cm Resistance, neutral site II integration platform for Synechococcus
    

    Use

    Cyanobacteria cloning vector

    Promoter

    n/a

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMTU-DO-HPH-C4

    Plasmid

    #160343

    Purpose

    High-copy expression vector for K. marxianus, hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_CD9-SBP-mCherry

    Plasmid

    #222329

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-mCherry (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGM65879

    Plasmid

    #153214

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pP2_tail_fiber

    Plasmid

    #196332

    Purpose

    Plasmid expressing P2 tail fiber H and chaperone protein G

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    P2 tail fiber H and chaperone protein G
    

    Expression

    Bacterial

    Promoter

    P2 late promoter

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLminP_Luc2P_RE38

    Plasmid

    #90378

    Purpose

    RELB - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    RELB

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. MSCV-Tox-IRES-eGFP

    Plasmid

    #165427

    Purpose

    Expresses TOX in mammalian cells

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX (Tox Mouse)
    

    Tags

    3xflag 5' of Tox

    Expression

    Mammalian

    Promoter

    5' LTR

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. Str-KDEL_CD9-SBP-EGFP

    Plasmid

    #222328

    Purpose

    Synchronize the trafficking of CD9 from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD9 fused to SBP-EGFP (CD9 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. Paxillin-pEGFP

    Plasmid

    #15233

    Depositor

    Rick Horwitz

    Article

    Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

    Insert

    paxillin (PXN Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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27. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. Mammalian expression: Z-lock cofilin dark state mutant

    Plasmid

    #141136

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. PaGFP-UtrCH

    Plasmid

    #26738

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    photoactivatable GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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