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pZDonor-Nm-Cas9-gRNA-hU6

Plasmid

#61366

Purpose

empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

Depositor

Charles Gersbach

Publication

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-Trastuzumab-IgM/κ

Plasmid

#61881

Purpose

Expresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

HER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette

Expression

Mammalian

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-AKAR4-Kras

Plasmid

#61621

Purpose

Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Publication

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4-Kras

Tags

C-terminal targeting sequence from Kras, Cerulean…

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DR274-eGFP sgRNA

Plasmid

#61051

Purpose

for generation of a eGFP specific sgRNA from a T7 promoter

Depositor

Filippo Del Bene

Publication

Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

Insert

EGFP sgRNA

Use

CRISPR

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICE-NLS-mCherry

Plasmid

#60364

Purpose

Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Publication

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

NLS-mCherry

Tags

NLS from SV40 T antigen and mCherry

Promoter

CMV-tet

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mEGFP-ER-5a

Plasmid

#56455

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

ER 5a (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP NLS Beta-Actin G13R

Plasmid

#60615

Purpose

Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

G13R

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGL3-human OCT4 PE-SV40-Luc

Plasmid

#52415

Purpose

Luciferase reporter plasmid for PE OCT4 enhancer activity

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Proximal element of hOCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Opto-mFGFR1_204

Plasmid

#58745

Purpose

Expresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Publication

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

Opto-mFGFR1

Expression

Mammalian

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Publication

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Publication

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Lifeact-FusionRed

Expression

Mammalian

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles
mEmerald-Gamma-Tubulin

Plasmid

#54105

Purpose

Localization: Centrosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mEmerald

Expression

Mammalian

Mutation

R47H

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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GSK3betaS9A (1016)

Plasmid

#49492

Purpose

expresses human Gsk3beta with S9A (constitutively active) mutation

Depositor

Scott Friedman

Publication

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta S9A (GSK3B Human)

Expression

Mammalian

Mutation

S9A (constitutively active)

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Publication

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iRFP-FRB-Rab7

Plasmid

#51613

Purpose

Recruiter to Rab7-positive endosomes

Depositor

Tamas Balla

Publication

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab7

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pACT2-CAS9c

Plasmid

#51055

Purpose

Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

Cas9c

Use

CRISPR

Tags

SV40 NLS

Expression

Bacterial and Yeast

Promoter

yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5-F-AF9

Plasmid

#49428

Purpose

Mammalian expression of Flag-tagged human AF9

Depositor

Joan Conaway, Ronald Conaway

Publication

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

AF-9 (MLLT3 Human)

Tags

Flag

Expression

Mammalian

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Publication

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Publication

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3xFLAG

Mutation

Serine 127 changed to alanine

Promoter

none

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLHCX-Flag-mPKM2

Plasmid

#42512

Depositor

Dimitrios Anastasiou

Publication

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2

Use

Retroviral

Tags

Flag

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Publication

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pHLUM

Plasmid

#40276

Depositor

Markus Ralser

Publication

Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

Inserts

LEU2 (LEU2 Budding Yeast)
URA3 (URA3 Budding Yeast)
MET15/17 (MET17 Budding Yeast)

Expression

Yeast

Promoter

endogenous LEU2, endogenous MET15/17, and endogen…

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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myc-NLRC5

Plasmid

#37509

Depositor

Thomas Kufer

Publication

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiN

Plasmid

#37444

Purpose

Control lentiviral plasmid containing FLAG-HA tags

Depositor

Karl Munger

Publication

Spangle et al J Virol. 2012 May 2.

Insert

FLAG-HA

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pcDNA3 HA Sam68 WT

Plasmid

#17690

Depositor

David Shalloway

Publication

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTAT2.1

Plasmid

#35620

Depositor

Steven Dowdy

Publication

Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

Type

Empty backbone

Tags

6xHIS and TAT

Expression

Bacterial

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
Dynactin-4/p62-GFP

Plasmid

#31071

Depositor

Vann Bennett

Insert

Dynactin-4 (DCTN4 Human)

Tags

EGFP

Expression

Mammalian

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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Arrb1-RFP

Plasmid

#35401

Depositor

Robert Lefkowitz

Publication

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

RFP

Expression

Mammalian

Promoter

T7

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article