We narrowed to 13,867 results for: cas9

1. LENTICRISPR-LUC7L_sgRNA2

   Plasmid

   #201601

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LENTICRISPR-LUC7L2_sgRNA2

   Plasmid

   #201603

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L2 (LUC7L2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LENTICRISPR-PABPC1_sgRNA2

   Plasmid

   #201609

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PABPC1 (PABPC1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pShuttle.CC9

   Plasmid

   #192931

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Promoter

   CMV

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-ALDH1A3gRNA3

   Plasmid

   #189748

   Purpose

   The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. phABCA3-eGFP_CRISPR_Donor

   Plasmid

   #188540

   Purpose

   Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   EGFP
   

   Use

   CRISPR; Donor for homologous recombination

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJET-osr1-KI-donor

   Plasmid

   #184875

   Purpose

   Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

   Depositor

   Christoph Winkler

   Article

   Hui et al Development. 2022 May 20. pii: 275483. d

   Inserts

   Odd-Skipped Related Transcription Factor 1 5' homology arm
   Odd-Skipped Related Transcription Factor 1 3' homology arm
   p2a-CreERT2
   Myosin light chain 2 promoter
   EGFP
   

   Use

   CRISPR and Cre/Lox

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX459_GRHL1-exon5

   Plasmid

   #177763

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459_GRHL1-exon8

   Plasmid

   #177764

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.U6gT28.4.hSyn.flex.H2B.RFP

    Plasmid

    #170386

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.U6gT28.3.hSyn.flex.H2B.RFP

    Plasmid

    #170374

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.U6gT28.3.hSyn.H2B.RFP

    Plasmid

    #170370

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.U6gT28.13.hSyn.flex.H2B.RFP

    Plasmid

    #170376

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.U6gT28.13.hSyn.H2B.RFP

    Plasmid

    #170372

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV.U6gT28.4.EFS-NS.H2B-RFP

    Plasmid

    #170365

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV.U6gT28.3.EFS-NS.H2B-RFP

    Plasmid

    #170364

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-uTEV1-MAVS-mTA2

    Plasmid

    #171000

    Purpose

    HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[R537A, R538A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-uTEV1-MAVS-mTA5

    Plasmid

    #171003

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI, R537A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-uTEV1-MAVS-mTA6

    Plasmid

    #171004

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-sgALCAM

    Plasmid

    #83929

    Purpose

    Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgALCAM
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-sgSLC35B2

    Plasmid

    #83932

    Purpose

    Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgSLC35B2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-sgTPST2

    Plasmid

    #83933

    Purpose

    Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgTPST2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-sgCTRL-1

    Plasmid

    #83934

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-sgCTRL-2

    Plasmid

    #83935

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-sgCTRL-3

    Plasmid

    #83936

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX459-sgPLXNB2

    Plasmid

    #86151

    Purpose

    Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

27. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSECC-sgSIK3-A

    Plasmid

    #138672

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSECC-sgSIK2-A

    Plasmid

    #138670

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSECC-sgSIK3-C

    Plasmid

    #138674

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSECC-sgSIK1-D

    Plasmid

    #138668

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSECC-sgSIK2-B

    Plasmid

    #138671

    Purpose

    Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 mouse (Sik2 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSECC-sgSIK1-E

    Plasmid

    #138669

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSECC-sgSIK3-D

    Plasmid

    #138675

    Purpose

    Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 mouse (Sik3 Mouse)
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AcrIIC1-mCherry chimera #10

    Plasmid

    #128113

    Purpose

    Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrIIC1-mCherry chimera #10
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1(-)-HF2-BE3

    Plasmid

    #120397

    Purpose

    Expresses HF2-BE3 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

    Insert

    HF2-BE3
    

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. sgGLDC_2

    Plasmid

    #72887

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

38. sgGLDC_1

    Plasmid

    #72886

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

39. A3A-PBE

    Plasmid

    #119768

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A and E945K in wild type …

    Promoter

    maize Ubiquitin-1

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge