20,128 results

Showing: 8641 - 8670 of 20128 results

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pLV-hCDC6p-Venus-puro

Plasmid

#212666

Purpose

Express Venus under CDC6 promoter

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Promoter

hCDC6p

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRS404-PTEF-Ago1

Plasmid

#22313

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

AGO1

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 NFAT3

Plasmid

#10961

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

NFAT3 (NFATC4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pSG5 PPAR alpha

Plasmid

#22751

Depositor

Bruce Spiegelman

Insert

PPARalpha (Ppara Mouse)

Expression

Mammalian

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5M-TIMP3

Plasmid

#31715

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TIMP3 (TIMP3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-HRP-TM_pLX304

Plasmid

#202021

Purpose

expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-HRP-TM

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo FlucSM EGFP

Plasmid

#90171

Purpose

EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

Depositor

Franz-Ulrich Hartl

Article

Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

Insert

FLuc EGFP

Expression

Mammalian

Mutation

R188Q in FLuc

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nup88

Plasmid

#64283

Purpose

GFP-tagged mammalian expression of full-length Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pKIKOlacZKm

Plasmid

#46767

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pclbw-eGFPomp25

Plasmid

#58424

Purpose

Mammalian expression of GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

eGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

CMV/b-actin

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Article

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

Plasmid

#29712

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Thioredoxin-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon DREADD Gi-mCherry

Plasmid

#177672

Purpose

Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

Depositor

Karl Deisseroth

Has Service

AAV8

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-P2A-HSA

Plasmid

#121936

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

dCas9-P2A-HSA

Use

CRISPR and Lentiviral

Tags

HSA

Expression

Mammalian

Promoter

CMV-TO

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T moesin 1-322

Plasmid

#11638

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

moesin (MSN Human)

Tags

GST

Expression

Bacterial

Mutation

Amino-terminal half of moesin.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pDM291-tetR'-tup11Δ70

Plasmid

#41028

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR'-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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Plenti-ARE-HSVTK-luc

Plasmid

#161789

Purpose

Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

Depositor

Hans-Guido Wendel

Article

Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

Inserts

Luciferase-T2A-HSV-TK
mCherry P2A blasticidin

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a and Murine Gclc ARE

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1

Plasmid

#33109

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD1 (TEAD1 )

Tags

Myc

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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HBx

Plasmid

#65463

Purpose

expresses HBx in mammalian cells

Depositor

Wang-Shick Ryu

Article

Cha et al Hepatology. 2004 Jun;39(6):1683-93.

Insert

HBV X protein

Tags

Flag

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoSuperfolderGFP-Kan

Plasmid

#44901

Purpose

Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoSuperfolderGFP

Expression

Yeast

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lwt

Plasmid

#74173

Purpose

For packaging hDot1Lwt to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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NPY-td-Orange2

Plasmid

#83497

Purpose

Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

Depositor

Sebastian Barg

Article

Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

Insert

NPY (NPY Human)

Tags

tandem mOrange2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Tags

3x-FLAG

Expression

Mammalian

Promoter

Lac

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-SV40

Plasmid

#117807

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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20,128 results