163,444 results

1. Flag-HA-USP5

   Plasmid

   #22590

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP5 (USP5 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX puro TRIM21-GFP

   Plasmid

   #116941

   Purpose

   Expression of TRIM21-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   TRIM21 - GFP (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-(-)-Pur

   Plasmid

   #200458

   Purpose

   Eukaryotic expression vector

   Depositor

   Bertrand Collet

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3/Flag-WTAP

   Plasmid

   #53741

   Purpose

   For expression of WTAP (Wilms tumor 1 associated protein) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Wilms tumor 1 associated protein (WTAP Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6a:sgRNA#2

   Plasmid

   #64246

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUGW-eGFP-ZEB1 3'UTR

   Plasmid

   #81018

   Purpose

   FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   Zeb1 3'UTR (UTS2R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. All-in-one lenti-evoCDA-NG

   Plasmid

   #200449

   Purpose

   Lentivirus vector for base editing

   Depositor

   Benjamin Cravatt

   Article

   Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6d:sgRNA#5

   Plasmid

   #64249

   Purpose

   expresses sgRNA under U6d promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PS6K-mcherry-iATPsnFR1.1

    Plasmid

    #227238

    Purpose

    Inducible ATP sensor for E. coli

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB402SC

    Plasmid

    #224552

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep-HA

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB402SN

    Plasmid

    #224551

    Purpose

    Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

    Depositor

    Paul Schulze-Lefert

    Article

    Lawson et al bioRxiv 2024.07.14.603419

    Type

    Empty backbone

    Use

    Tags

    HA-Twin-Strep

    Expression

    Plant

    Mutation

    Promoter

    2 X 35S

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-ADRBK1

    Plasmid

    #23835

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ADRBK1 (GRK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX403-ccdB-Blast

    Plasmid

    #158560

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Richard Youle, Alexander van der Bliek

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Use

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5F-TACE

    Plasmid

    #31713

    Purpose

    Mammalian expression of human TACE (ADAM17)

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TACE (ADAM17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge