165,947 results

1. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LM627

   Plasmid

   #191551

   Purpose

   LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Tags

   SNAC-tag, HIS-tag

   Expression

   Bacterial

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-S5E2-dTom-nlsdTom

   Plasmid

   #135630

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV PHP.eB, AAV1, and AAV9

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Mutation

   None

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lentiviral CaMKKbeta

   Plasmid

   #33322

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Promoter

   human PGK

   Available Since

   April 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lentiviral CaMKKbeta K193A

   Plasmid

   #33323

   Depositor

   Anthony Means

   Article

   Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

   Insert

   Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   K194A

   Promoter

   human PGK

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATeam1.03-nD/nA/pcDNA3

   Plasmid

   #51958

   Purpose

   mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

   Depositor

   Takeharu Nagai

   Article

   Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

   Insert

   ATeam1.03-nD/nA
   

   Expression

   Mammalian

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH47742::p35S-ePEmax3::tEURb7

   Plasmid

   #227692

   Purpose

   Golden Gate level 1, position 2, ePEmax3 expression cassette.

   Depositor

   Jae-Yean Kim

   Article

   Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

   Insert

   ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-mCherry-(F30-2xPepper)10

   Plasmid

   #129401

   Purpose

   mCherry mRNA reporter tagged with (F30-2xPepper)10

   Depositor

   Samie Jaffrey

   Article

   Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

   Insert

   mCherry-(F30-2xPepper)10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVone-AAV2-CMV-eGFP

    Plasmid

    #230930

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Article

    Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-KRAS-G12D

    Plasmid

    #116423

    Purpose

    Lentiviral expression of KRAS G12D

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Mutation

    G12D

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLH01

    Plasmid

    #117261

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-Lp_0640-42, sapK-sappR
    

    Use

    Other

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

    Plasmid

    #125712

    Purpose

    Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    eOPN3
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 331-429

    Promoter

    short version of αCamKinase II promoter (0.4kb)

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLSL.R.Ly

    Plasmid

    #227697

    Purpose

    Reengineered geminiviral replicon vector

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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