We narrowed to 23,940 results for: nes

1. pGFP-nE-mHP1alpha

   Plasmid

   #181895

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92390

   Purpose

   expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200402

   Purpose

   Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

   Plasmid

   #130816

   Purpose

   Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   bJun-NFAST-NLS-DEVDG-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

   Plasmid

   #173872

   Purpose

   Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

   Plasmid

   #173871

   Purpose

   Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

   Plasmid

   #120555

   Purpose

   Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   Cas9, NES, ERT2 and GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

    Plasmid

    #42046

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

    Plasmid

    #101063

    Purpose

    AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2_F391W-G395D
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

    Plasmid

    #125225

    Purpose

    Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

    Plasmid

    #196831

    Purpose

    Express PUFc-RESCUE-S

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    PUFc-RESCUE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmyc-GFP-TNRC6A-(935-1026)-NES-mut

    Plasmid

    #42021

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-1026, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

    Plasmid

    #173875

    Purpose

    Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

    Plasmid

    #173876

    Purpose

    Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101101

    Purpose

    Encodes dCas9(N) fused to transmembrane tether

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    TMt-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

    Plasmid

    #42040

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa457-532, aa716-772 and aa804-924, F951A…

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge