We narrowed to 30,440 results for: uros

1. px459-RhebL1 sgRNA

   Plasmid

   #133769

   Purpose

   Expresses Cas9 and human RhebL1 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CP1028-ABE8e-dimer

   Plasmid

   #138498

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-SP-CLSTN2-3xHAtag

   Plasmid

   #191693

   Purpose

   For expression coding sequencing of CLSTN2

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MAP2-CD4

   Plasmid

   #43916

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human MAP2 promoter

   Available since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGWB528

   Plasmid

   #74870

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-EF1a-V5-Sec61B

   Plasmid

   #120244

   Purpose

   Expresses V5-tagged Sec61B in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Sec61B (Sec61b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSSA-PMG (pXK-1649)

   Plasmid

   #208857

   Purpose

   SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

   Depositor

   Kun Xu

   Article

   Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLib6.4-Aalb_744

   Plasmid

   #176663

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GWF180

   Plasmid

   #133607

   Purpose

   CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

   Depositor

   Dustin Maly

   Article

   Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

   Insert

   DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hST3GAL6-RFP

    Plasmid

    #22923

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQZ083

    Plasmid

    #106417

    Purpose

    Express Complexin-1 fragment (1-83) in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Insert

    complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Fragment 1-83, Codon optimization

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

    Plasmid

    #190875

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode206
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDC184

    Plasmid

    #70055

    Purpose

    Co-expresses Syt1-SNARE37aa-linker complex with p045

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

    Inserts

    His tagged Synaptobrevin 28-89
    Syntaxin 191-256
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB419

    Plasmid

    #74813

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-mBAF45b IRES GFP

    Plasmid

    #17881

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45b (Dpf1 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

19. FLAG-mBAF45c IRES GFP

    Plasmid

    #17882

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45c (Dpf3 Mouse)
    

    Use

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

20. GFP-GLT1a-V5

    Plasmid

    #162517

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p-715MRPprom-MRPcucuc

    Plasmid

    #87199

    Purpose

    Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    RMRP (RMRP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GAGAG91-95 to CUCUC

    Promoter

    human RMRP

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGWB522

    Plasmid

    #74864

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-GLT1b-V5

    Plasmid

    #162518

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSLQ1371-sgSTAT3-1

    Plasmid

    #121425

    Purpose

    sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgSTAT3-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Myc-Foxo1-WT 6KQ

    Plasmid

    #17559

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KQ (Foxo1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pLKO.1p shDna2

    Plasmid

    #31951

    Depositor

    Sheila Stewart

    Article

    Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

    Insert

    shRNA against hDna2 (DNA2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

27. PKC-delta-IRES-hrGFP

    Plasmid

    #73414

    Purpose

    Mammalian expression of mouse PKC-delta and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKC-delta (Prkcd Mouse)
    

    Use

    Tags

    3xFLAG and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

28. L40C-CRISPR.EFS.PAC

    Plasmid

    #89393

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p-mCherry-N1-iChloC

    Plasmid

    #85467

    Purpose

    improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse)

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Insert

    iChloC
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, T159C, D156N

    Promoter

    CMV (+enhancer)

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLib6.4-Cquin_596

    Plasmid

    #176655

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBS-TRE-mTagBFP2-WPRE

    Plasmid

    #193332

    Purpose

    Expresses mTagBFP2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A XhoI site in the coding sequence is silenced

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CAG-EGFP barcode-10-SV40 polyA

    Plasmid

    #190874

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV-EF1a-GFP RanSen

    Plasmid

    #184705

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCETN1-IRFP670

    Plasmid

    #99879

    Purpose

    Expression of CETN1-iRFP670 in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    CETN1 (CETN1 Human)
    

    Use

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pLX317 PRDM14

    Plasmid

    #193682

    Purpose

    Constitutive lentiviral expression of PRDM14

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFUW-tetO-Zbtb46

    Plasmid

    #139811

    Purpose

    doxycycline-inducible expression of mouse Zbtb46 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Zbtb46 (Zbtb46 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pTYF-super-mfPV-0.8k-Venus-WPRE

    Plasmid

    #156399

    Purpose

    Expresses Venus under PV gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge