We narrowed to 21,470 results for: SPR
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Plasmid#176527PurposeDelivers all prime editing nickase components in a single plasmidDepositorInsertCbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseCRISPRExpressionMammalianPromoterCbH for Cas9, hU6 for gRNAsAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_NPM1_G6
Plasmid#178091PurposeExpresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.DepositorInsertNPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Puro-sgAMPKa1
Plasmid#138684PurposeExpresses a human AMPKa1-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK375 (Hygro-P2A-Stag-3FLAG)
Plasmid#121186PurposeN-terminal taggingDepositorInsertHygro-P2A-Stag-3FLAG
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATF4.1.0-gDNA
Plasmid#113772PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4DepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-KKHSaCas9-BlastR
Plasmid#107189PurposeConfers constitutive expression of KKH SaCas9.DepositorInsertKKH SaCas9 (NEWENTRY )
ExpressionBacterialMutationE782K/N968K/R1015HPromoterChicken ß-ActinAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLTA-mCherry HDRT Source (pTR 177)
Plasmid#112017PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherryDepositorInsertCLTA-mCherry HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
TagsHis6 tagExpressionBacterialPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRExpressionYeastPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME464
Plasmid#238068PurposeExpressed ME-ABE ABE7.10-HRHSK with SpRYCas9DepositorInsertTadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include L36H, L51R, Y123H, C14…Available SinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS--GFP-p300
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-AAVS1_sgRNA
Plasmid#183890PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
ExpressionMammalianPromoterU6Available SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas12f-GE ver3.0
Plasmid#176508PurposeExpresses Cas12f-GE in mammalian cellsDepositorInsertsCas12f-GE ver3.0
Cas12f-GE ver3.0
ExpressionMammalianPromoterU6 and chicken β-actinAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
px459-TDP 43 sgRNA2
Plasmid#133763PurposeExpresses Cas9 and human TDP-43 sgRNADepositorAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-PGK-Puro
Plasmid#110860PurposeLentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
SunTagCACTA1g2-22aa-TET1cd
Plasmid#106437PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoterDepositorInsertCACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA SOX17 -177
Plasmid#50925PurposeU6 driven sgRNA targeting Sox17 -177 bp from TSSDepositorAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only