We narrowed to 30,619 results for: ica

1. NAX_CAGGS_CAS9_KanR neo

   Plasmid

   #167861

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pACS130

   Plasmid

   #204595

   Purpose

   Histone H3.1K9M-V5

   Depositor

   Tom Misteli

   Article

   Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

   Insert

   histone H3.1 (H3C3 Human)
   

   Use

   Lentiviral

   Tags

   human histone H3.1K9M-V5

   Expression

   Mammalian

   Mutation

   H3.1K9M

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

   Plasmid

   #87644

   Purpose

   For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   myr::SNAPf>myr::CLIPf>myr::Halo2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB-bsd-Flag-Trl1

   Plasmid

   #126609

   Purpose

   Expresses N-terminal Flag-tagged yeast Trl1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA ligase 1 (TRL1 Budding Yeast)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CROP_C9_Puro_PDGFRA

   Plasmid

   #183313

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets PDGFRA gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   PDGFRA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-C1-hRabin8 F201A

   Plasmid

   #26728

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Insert

   Rabin8 (RAB3IP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   F201A: GEF domain mutant

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pVAX-Help-SIINFEKL

   Plasmid

   #141350

   Purpose

   DNA vaccination plasmid containing SIINFEKL and several CD4+ T cell epitopes

   Depositor

   Ton Schumacher

   Article

   Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

   Insert

   HELP-SIINFEKL-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pORE303N_CEN12RRFuzzy3MYB

   Plasmid

   #194441

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, RR, Fuzzy3MYB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-rab5c-DN

   Plasmid

   #109737

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative rab5c

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab5c (rab5c Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   dominant negative

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1-hRabin8 L196A

    Plasmid

    #26727

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L196A: GEF domain mutant.

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

11. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUAS-hActin-luc2

    Plasmid

    #48081

    Purpose

    Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    removed hsp70 promoter, inserted amplified 615 bp…

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

13. SLF091: Lenti-CaMKIIa-eTsChR-eGFP

    Plasmid

    #112281

    Purpose

    For lentiviral or direct expression of eTsChR in excitatory neurons

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    eTsChR
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROP_C9_Puro_JAK2

    Plasmid

    #183303

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    JAK2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-myrSNAP-CON

    Plasmid

    #60099

    Purpose

    Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

    Depositor

    Gregory Jefferis

    Article

    Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

    Inserts

    SNAP-tag
    histone-GFP
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC-SARS-CoV-2 Orf9c

    Plasmid

    #158379

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 Orf9c
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. EFS eGFP hPGK BSD

    Plasmid

    #188520

    Purpose

    Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JB93

    Plasmid

    #128904

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab7AQL-BirA*-HA-MAO (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pME18S-3HA-mULK1(K46N)

    Plasmid

    #22897

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Biochem Biophys Res Commun. 1998 May 8.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Aspargine

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

20. Danio rerio cryabb

    Plasmid

    #47665

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha B-crystallin (cryabb Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mEmerald-MCM6-N-22

    Plasmid

    #54172

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

    Insert

    MCM6
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pWXLd-RIEP-OgNLuc

    Plasmid

    #135936

    Purpose

    Expresses the OgNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046879

    Plasmid

    #149238

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pHAGE- TRE-DEST-CBioTAP

    Plasmid

    #53569

    Purpose

    Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

    Depositor

    Mitzi Kuroda

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-GFP-2AFP-KDEL

    Plasmid

    #154349

    Purpose

    Mammalian expression of 2AFP

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2AFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.CAG-FLEX.iGABASnFR.F102G

    Plasmid

    #112167

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW4.02

    Plasmid

    #62867

    Purpose

    Bacterial expression of MBP-PGL-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-PGL-1
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

29. actc1b-mKate2-CaaX(tH)

    Plasmid

    #109487

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-CaaX(tH)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGBW-m4046942

    Plasmid

    #145757

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. JB19

    Plasmid

    #128913

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab11ASN-BirA*-HA-MAO (RAB11A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3.1-EPSIN 1

    Plasmid

    #22225

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    Xpress

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pMito.iGABASnFR.R205A

    Plasmid

    #112178

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R205A (no GABA binding)

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKMV-VioE

    Plasmid

    #65351

    Purpose

    systhesis of vioE gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pADH139

    Plasmid

    #90987

    Purpose

    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    gRNA conserved, C, mal LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pNOC-CRISPR-HA

    Plasmid

    #99370

    Purpose

    Expresses Cas9-HA and sgRNA. Hygromycin resistance.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-HA

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HS-Utrophin-GFP

    Plasmid

    #105952

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Utrophin (Utrn Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT7-Venus-Emi1 (914)

    Plasmid

    #39854

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

    Insert

    Emi1 (FBXO5 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MTK1_008

    Plasmid

    #123671

    Purpose

    Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge