We narrowed to 18,361 results for: met

1. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

   Plasmid

   #52054

   Purpose

   Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

   Depositor

   Jesse Rinehart

   Inserts

   SepRS
   EF-Sep
   5x tRNA Sep
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

   Promoter

   Ptac

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT EGFP-miniAID

   Plasmid

   #101714

   Purpose

   For amplification of a EGFP-miniAID for N-terminal tagging

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   EGFP-miniAID
   

   Use

   Tags

   EGFP-miniAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXD80RFP-pXD68Kan2-RFP

   Plasmid

   #225329

   Purpose

   RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. BoNT/A-LC

   Plasmid

   #31602

   Depositor

   Axel Brunger

   Article

   Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

   Insert

   BoNT/A LC
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMEG_YLCas9_empty

   Plasmid

   #117826

   Purpose

   CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

   Depositor

   Michael Sauer

   Article

   Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

   Insert

   YlpTEF_hCas9_YlCyc1TT
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet1_CylLS_CylM

   Plasmid

   #208760

   Purpose

   Expresses modified cytolysin S (mCylLS) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CylLS
   CylLM
   

   Use

   Tags

   Hisx6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-663 (attR4-attR2)

   Plasmid

   #161884

   Purpose

   Multisite Gateway (attR4-attR2) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET FLIPPi-5u

   Plasmid

   #13554

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi S52A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   S52A in binding protein

   Promoter

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEZY19

   Plasmid

   #18668

   Purpose

   pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAH121

    Plasmid

    #164901

    Purpose

    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Insert

    phi21 Integrase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTETDuet_M42FG121VDHFR_R166QTS

    Plasmid

    #191298

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M42F/G121V DHFR, R166Q TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMF037

    Plasmid

    #157634

    Purpose

    Batis maritima MHT

    Depositor

    Jonathan Silberg

    Article

    Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

    Insert

    MHT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MXS_chaining vector

    Plasmid

    #62394

    Purpose

    Empty Backbone

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV::ratSyGCaMP2

    Plasmid

    #26124

    Depositor

    Leon Lagnado

    Article

    Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

    Insert

    Synaptophysin GCaMP2 (Syp Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEERM1 Km

    Plasmid

    #64024

    Purpose

    Plasmid for integration into psbA2 site of Synechocystis 6803

    Depositor

    Pia Lindberg

    Article

    Englund et al ACS Synth Biol. 2015 Jul 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PpsbA2

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInh

    Plasmid

    #199998

    Purpose

    Inhibitor of transposase and integrase

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTYB2-eIF4B

    Plasmid

    #37226

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pRS424GFP-2xPH(Osh2)

    Plasmid

    #36095

    Depositor

    Scott Emr

    Article

    Stefan et al Cell. 2011 Feb 4;144(3):389-401.

    Insert

    2xPH(Osh2) fusion
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PRC1

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pUK

    Plasmid

    #64185

    Purpose

    harbours marker genes URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB3nK_ext_AC

    Plasmid

    #108686

    Purpose

    Plasmid for assembly of donor DNA templates consisting of two expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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