We narrowed to 19,041 results for: Cre
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Plasmid#215917PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut1-SM
Plasmid#215918PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut2-SM
Plasmid#215919PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT&Mut-CM
Plasmid#215920PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_WT-CM
Plasmid#215921PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_ WT (BRAF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut1-CM
Plasmid#215922PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-BRAF_Mut2-CM
Plasmid#215923PurposeThis plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.DepositorInsertone complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)
UseTagsExpressionMammalianMutationBRAFPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control-TP53-LRG-GFP
Plasmid#225874PurposeGuides targeting P53 and a control sequenceDepositorInsertControl and TP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control-RB1-LRG-GFP
Plasmid#225875PurposeGuides targeting RB1 and a control sequenceDepositorInsertControl and RB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion
Plasmid#224388PurposeLenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell linesDepositorInsertGNB1L (GNB1L Human)
UseTagsExpressionBacterial and MammalianMutationWD 7 domain deleted (aa 286-323)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion
Plasmid#224387PurposeLenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell linesDepositorInsertGNB1L (GNB1L Human)
UseTagsExpressionBacterial and MammalianMutationWD 6 domain deleted (aa 242-282)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion
Plasmid#224385PurposeLenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell linesDepositorInsertGNB1L (GNB1L Human)
UseTagsExpressionBacterial and MammalianMutationWD 4 domain deleted (aa 153-195)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8
Plasmid#209050PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-miniTurbo-TAF5-∆ex8
Plasmid#209052PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsminiTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-FL_WobbleMut
Plasmid#209055PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut
Plasmid#209056PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic-As
Plasmid#209036PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-nSpCas9-TadA8.20m
Plasmid#209045PurposeLentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLSDepositorInsertTadA8.20m-nSpCas9
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationD10APromoterTight TRE promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic_2-As
Plasmid#218814PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic_3-As
Plasmid#218815PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic_4-As
Plasmid#218816PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
Plasmid#218973PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)DepositorInsertTAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
UseTagsExpressionMammalianMutationmutant SRSF1 binding motif (AGTCTCCT)PromoterRSV LTRAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR1-DuET
Plasmid#213183PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAGTR1 (AGTR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-113-117-5A
Plasmid#213643PurposeExpresses human SKP1-113-117-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-113-117-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-138-142-5A
Plasmid#213645PurposeExpresses human SKP1-138-142-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-138-142-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-120-124-5A
Plasmid#213644PurposeExpresses human SKP1-120-124-5A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-120-124-5A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-RR (K142R/K163R)
Plasmid#213425PurposeExpresses human SKP1 K142R/K163R mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-K142R/K163R (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-BCL-XL-499R
Plasmid#203574PurposeExpresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.DepositorInsertBCL2L1 (BCL2L1 Human)
UseLentiviralTagsV5- taggedExpressionMammalianMutationsilent point mutations introduced to make it resi…PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-SKP1-F139A/I141A
Plasmid#213687PurposeExpresses human SKP1-F139A/I141A mutant in mammalian cellsDepositorInserthuman Flag tagged SKP1-F139A/I141A (SKP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-E993A
Plasmid#203698Purposeexpresses ATP10B with the pathogenic mutation E993ADepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationE993APromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-E210A
Plasmid#203697Purposeexpresses ATP10B with the catalytic mutation E210ADepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationE210APromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-D433N
Plasmid#203696Purposeexpresses ATP10B with the catalytic mutation D433NDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationD433NPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-V748L
Plasmid#203702Purposeexpresses ATP10B with the pathogenic mutation V748LDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationV748LPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-I1038T
Plasmid#203700Purposeexpresses ATP10B with the pathogenic mutation I1038TDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationI1038TPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ATP10B-R153X
Plasmid#203701Purposeexpresses ATP10B with the pathogenic mutation R153XDepositorInsertATP10B (ATP10B Human)
UseTagsExpressionMammalianMutationR153XPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ERAP1-rs27895(T)
Plasmid#206143PurposeExpresses the rs27895-T allele of ERAP1, myc-tagged.DepositorInsertERAP1 (ERAP1 Human)
UseTagsmycExpressionMutationG346 changed to D346PromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1101
Plasmid#143925PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2IRD1 (GTF2IRD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1100
Plasmid#143924PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2IRD1 (GTF2IRD1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pExp-His-zBasic-RBD-Avi
Plasmid#195000PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsAvi-tag and His8-ZbasicExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187611PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationPromoterEF1AAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only