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pSum-LacZ-gfp

Plasmid

#109285

Purpose

Expresses gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

GFP

Expression

Bacterial

Mutation

GFP modified for expression in prokaryotes; inclu…

Promoter

LacZalpha

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMB-SaCas9

Plasmid

#105998

Purpose

SaCas9 expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Insert

Cas9 nuclease

Use

CRISPR and Lentiviral

Tags

none

Expression

Mammalian

Mutation

none

Promoter

short EF1alpha

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a1

Plasmid

#13754

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET32a-hTGFa

Plasmid

#199236

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Transforming growth factor alpha, partial (TGFA Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro LEF1

Plasmid

#27023

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

LEF1 (LEF1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pEF-XMAS-1xStop

Plasmid

#164411

Purpose

Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

T2A-GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:Renilla:Tnos (GB0109)

Plasmid

#68215

Purpose

Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDestattB/CY (pCM327)

Plasmid

#68341

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Article

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Insert

Bmi1 (BMI1 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-Low-CAR_RHL001

Plasmid

#164824

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-Low CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Promoter

SFFV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-DRD2

Plasmid

#24099

Depositor

Jean-Michel Arrang

Article

Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

Insert

DRD2 (DRD2 Human)

Tags

alpha7 nicotinic signal peptide and eGFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::FLuc

Plasmid

#178316

Purpose

P53 Firefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

P53 FLuc Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-SN

Plasmid

#181741

Purpose

AAV vector for expressing SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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HA PKB T308D S473D pcDNA3

Plasmid

#14751

Depositor

Jim Woodgett

Article

Scheid et al Mol Cell Biol. 2002 Sep . 22(17):6247-60

Insert

PKB DD (AKT1 Bovine)

Tags

HA

Expression

Mammalian

Mutation

T308D S473D. Constitutively active mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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