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We narrowed to 11,980 results for: SOM

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Showing: 11881 - 11920 of 11980 results

  1. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid
    #210024
    Purpose
    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    Expression
    Mammalian
    Mutation
    Encephalomyocarditis virus internal ribosome entr…
    Promoter
    CMV
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210024-plasmid-map-sequence-id-417730

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  2. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

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  3. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  5. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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  7. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Insert
    GFP-Gal8 (LGALS8 Human)
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127191-plasmid-map-sequence-id-248359

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  8. IronFistMUT.

    Plasmid
    #243008
    Purpose
    Iron binding deficient IronFist
    Depositor
    Roland Malli
    Article
    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d
    Inserts
    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    Use
    Gateway
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    H15A, H57A, E58A, E61A, H126A, E130A
    Promoter
    human phosphoglycerate kinase (hPGK)
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    243008-plasmid-map-sequence-id-486388

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  13. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  14. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  15. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  16. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  18. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid
    #26797
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    Rs1 (HTR4 Human)
    tTA
    Tags
    mCherry-P2A
    Expression
    Mammalian
    Mutation
    D100A
    Promoter
    EF1α and short TetO, miniCMV
    Available Since
    Feb. 23, 2011
    Availability
    Academic Institutions and Nonprofits only
    26797-plasmid-map-sequence-id-12685

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  19. pWZ194

    Plasmid
    #163640
    Purpose
    rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Promoter
    rps-27
    Available Since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    163640-plasmid-map-sequence-id-324064

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  20. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid
    #24419
    Purpose
    Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration.
    Depositor
    Edward Hsiao
    Article
    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
    Inserts
    RS1 (HTR4 Human)
    tTA
    Use
    Mouse Targeting
    Tags
    FLAG and mCherry-P2A
    Mutation
    D100A
    Promoter
    EF1α and TRE, miniCMV
    Available Since
    March 14, 2011
    Availability
    Academic Institutions and Nonprofits only
    24419-plasmid-map-sequence-id-11416

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  22. pAWK61

    Plasmid
    #163642
    Purpose
    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Human, Nematode)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Promoter
    rps-27
    Available Since
    May 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    163642-plasmid-map-sequence-id-362086

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  27. pTB106-Hyg

    Plasmid
    #236779
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and hygromycin selection marker. The CBE contains a ssDNA-DBD from the L. major RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236779-plasmid-map-sequence-id-494138

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  28. pTB107-Phleo

    Plasmid
    #236782
    Purpose
    Expression of Cas9 cytosine base editor (CBE), T7 RNAP, Cas12a and phleomycin selection marker. The CBE contains a ssDNA-DBD from the H. sapiens RAD51 protein. This plasmid is transfected as episome.
    Depositor
    Tom Beneke
    Article
    Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7
    Insert
    hyBE4max (CBE); Cas12a (Acidaminococcus sp. BV3L6)-P2A-T7 RNAP
    Use
    CRISPR
    Available Since
    Dec. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236782-plasmid-map-sequence-id-494137

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  30. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

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  31. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  39. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107999-plasmid-map-sequence-id-217151

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Showing: 11881 - 11920 of 11980 results