2,226 results

1. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti sfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164478

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-LAMP1-phLuorin-N-8

   Plasmid

   #54918

   Purpose

   Localization: Lysosome Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   LAMP1 phLuorin
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Lysosomes-20 double labeled with phLuorin

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GLUT1-GFP

   Plasmid

   #89440

   Purpose

   GLUT1 construct

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

   Insert

   GLUT1 (Slc2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mOrange2-Lysosomes-20

   Plasmid

   #57968

   Purpose

   Localization: Lysosome Membrane, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

   Plasmid

   #131507

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSYN1-NES-OCaMP-WPRE

   Plasmid

   #224934

   Purpose

   pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

   Depositor

   Alexander Lohman

   Article

   Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

   Insert

   OCaMP
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSynI

   Available since

   July 28, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AAV-hSyn-RiboL1-jGCaMP8s

   Plasmid

   #169247

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CMV-NES-jRCaMP1a

   Plasmid

   #61562

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1a
   

   Use

   Tags

   nuclear export signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myo 9a-GFP

    Plasmid

    #134959

    Purpose

    Expression of rat Myo 9a (Myr7) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Chieregatti et al J Cell Sci. 1998 Dec 18;111 ( Pt 24):359

    Insert

    Myo 9a (rat) with 5' UTR (Myo9a Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Myo 1c

    Plasmid

    #134832

    Purpose

    Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

    Depositor

    Martin Bähler

    Article

    Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

    Insert

    Myo 1c (rat) with 3' UTR (Myo1c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-αIFNα-ab

    Plasmid

    #110799

    Purpose

    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells

    Depositor

    Thomas Böldicke

    Article

    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

    Insert

    antibody against mouse interferon alpha
    

    Use

    Tags

    ER signal peptide, His6 tag, and myc epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Myo 9b

    Plasmid

    #134907

    Purpose

    Expression of rat Myo 9b (Myr 5) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

    Insert

    Myo 9b (rat) with 3' UTR (Myo9b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

    Plasmid

    #102935

    Purpose

    An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    NK1R (Tacr1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Myo1b

    Plasmid

    #187362

    Purpose

    Expresses rat myosin 1b isoform b labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

    Insert

    myosin 1b isoform b (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-αIFNα-ib

    Plasmid

    #110798

    Purpose

    Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

    Depositor

    Thomas Böldicke

    Article

    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

    Insert

    intrabody against mouse interferon alpha
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK292 (mAID-mCherry2-NeoR)

    Plasmid

    #72830

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Neo
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSynapsin_NLS_GFP_P2A_His6_NR1.

    Plasmid

    #170457

    Purpose

    Expresses histidine tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    Hexa-histidin

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. myc-mTOR

    Plasmid

    #1861

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-TRSter

    Plasmid

    #78865

    Purpose

    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

    Depositor

    Elias Arnér

    Article

    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

    Insert

    Thioredoxin Reductase (Txnrd1 Rat)
    

    Use

    Tags

    Mutated E. coli-type SECIS element (3’ end of ins…

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLJC5-LAMP1-RFP-3xHA

    Plasmid

    #102932

    Purpose

    Express a lysosomal Tag

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    RFP-HA

    Expression

    Mutation

    Promoter

    ubc

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Epsin1 deltaUIM-ECFP

    Plasmid

    #20973

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Epsin1 deltaUIM (Epn1 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2009

    Availability

    Academic Institutions and Nonprofits only

26. Epsin1-ECFP

    Plasmid

    #21066

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pEYFP-NR1a

    Plasmid

    #17928

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGP-CMV-NES-jRCaMP1b

    Plasmid

    #63136

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1b
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge