Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 770 results for: NEB

Showing: 81 - 120 of 770 results

  5. pCVD30

    Plasmid
    #63691
    Purpose
    pCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.
    Depositor
    James Kaper
    Article
    Kaper et al Nature Biotechnology 2, 345 - 349 (1984)
    Insert
    V. cholera chromosomal DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    540 bp of CT A is deleted
    Promoter
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pTXB1-CRP/CAP

    Plasmid
    #51563
    Purpose
    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    CRP/CAP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51563-plasmid-map-sequence-id-78803

    Enlarge

  23. pUBC_HA_L7Ae-VP64

    Plasmid
    #68421
    Purpose
    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    L7Ae
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    Promoter
    human UbC
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68421-plasmid-map-sequence-id-124596

    Enlarge

  27. pGBW-m4092694

    Plasmid
    #153907
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153907-plasmid-map-sequence-id-307331

    Enlarge

  28. pGBW-m4092709

    Plasmid
    #153859
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Nearly native encoding;handful SNPs as watermarks…
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153859-plasmid-map-sequence-id-301594

    Enlarge

  32. pGJJ055

    Plasmid
    #183622
    Purpose
    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183622-plasmid-map-sequence-id-361477

    Enlarge

  36. NSm-GFP pZK538

    Plasmid
    #118012
    Purpose
    expresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).
    Depositor
    Xiaorong Tao, FLW Takken
    Article
    et al Bio-protocol 8(24): e3114.
    Inserts
    NSm
    mCherry
    Use
    Tags
    EGFP and HDEL
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    35S
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    118012-plasmid-map-sequence-id-233232

    Enlarge

Showing: 81 - 120 of 770 results