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Showing: 101 - 150 of 383 results

  25. CROPSeq-PP7-p65-HSF

    Plasmid
    #196719
    Purpose
    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196719-plasmid-map-sequence-id-388066

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  31. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  32. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  34. pcDNA3-ABKAR

    Plasmid
    #140051
    Purpose
    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.
    Depositor
    Jin Zhang
    Article
    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4
    Insert
    ABKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140051-plasmid-map-sequence-id-307466

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  42. pCAG MCP-VP64

    Plasmid
    #92357
    Purpose
    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    MCP-VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92357-plasmid-map-sequence-id-178154

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Showing: 101 - 150 of 383 results