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pCYP17

Plasmid

#212850

Purpose

Expresses the Actinomadura sp. KC06 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID

Plasmid

#209386

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

TurboID gene sequence C249A

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID-GFP

Plasmid

#209387

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

TurboID gene sequence C249A

Promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LI B-C TurboID

Plasmid

#209385

Purpose

Golden Gate LI B-C module

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

TurboID gene sequence C249A

Promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBSII-flotillin1c

Plasmid

#62801

Purpose

enriched expression in dorsal neural tube

Depositor

Sally Moody

Article

Pandur et al Dev Dyn. 2004 Dec;231(4):881-7.

Insert

flotillin 1c (flot1-b Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pJet1.2 HA6-Tag

Plasmid

#117126

Purpose

C-terminal HA-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xHA Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xHA tag

Expression

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Pk6-tag

Plasmid

#117127

Purpose

C-terminal Pk6-tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

6xPK tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

6xPK tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 'rncS

Plasmid

#117129

Purpose

smc gene upstream homology region

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

'rncS

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS09

Plasmid

#67160

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS09

Use

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

L414T within GCaMP6f

Promoter

T7

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 ermB cassette

Plasmid

#117121

Purpose

Erythromycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Erythromycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 kanR cassette

Plasmid

#117122

Purpose

Kanamycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Kanamycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 cat cassette

Plasmid

#117119

Purpose

Chloramphenicol resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Chloramphenicol resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJet1.2 specR cassette

Plasmid

#117120

Purpose

Spectinomycin resistance cassette

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Spectinomycin resistance cassette for Bacillus subtilis selection

Use

Golden gate donor vector for gene targeting in ba…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSLICER

Plasmid

#197288

Purpose

Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

Depositor

Bogumil Karas

Article

Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

Insert

I-SceI Endonuclease

Use

Synthetic Biology; Bacterial and yeast cloning

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Use

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Avi-tag

Plasmid

#117128

Purpose

C-terminal Tev-Avi tag Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev Avi-tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev Avi-Tag

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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