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We narrowed to 2,326 results for: dcas9

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Showing: 1161 - 1200 of 2326 results

  2. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  3. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  4. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50587-plasmid-map-sequence-id-75659

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  5. MT-AvrXa10-FT

    Plasmid
    #234373
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus
    Depositor
    Michael Smanski
    Article
    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202
    Inserts
    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    Use
    Synthetic Biology
    Tags
    GB1, GFP, and GP41
    Expression
    Plant
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234373-plasmid-map-sequence-id-469429

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  6. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50579-plasmid-map-sequence-id-75626

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  7. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50580-plasmid-map-sequence-id-75637

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  8. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

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  9. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  10. pWJ66

    Plasmid
    #46570
    Purpose
    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9-w
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46570-plasmid-map-sequence-id-65846

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  11. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  12. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  13. PLJR965_Rv3297

    Plasmid
    #241235
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3297 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241235-plasmid-map-sequence-id-483383

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  14. PLJR965_Rv2976c

    Plasmid
    #241234
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2976c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241234-plasmid-map-sequence-id-483384

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  15. PLJR965_Rv2464c

    Plasmid
    #241232
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2464c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241232-plasmid-map-sequence-id-483381

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  16. PLJR965_Rv1638

    Plasmid
    #241231
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1638 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241231-plasmid-map-sequence-id-483389

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  17. PLJR965_Rv1259

    Plasmid
    #241228
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1259 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241228-plasmid-map-sequence-id-483385

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  18. PLJR965_Rv1020

    Plasmid
    #241227
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1020 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241227-plasmid-map-sequence-id-483388

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  19. PLJR965_Rv0949

    Plasmid
    #241226
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv0949 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241226-plasmid-map-sequence-id-483386

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  20. PLJR965_Rv1633

    Plasmid
    #241230
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1633 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241230-plasmid-map-sequence-id-483390

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  21. PLJR965_Rv3589

    Plasmid
    #241236
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3589 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241236-plasmid-map-sequence-id-483387

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  22. PLJR965_Rv2924c

    Plasmid
    #241233
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2924c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241233-plasmid-map-sequence-id-483382

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  23. PLJR965_Rv1420

    Plasmid
    #241229
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1420) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1420 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241229-plasmid-map-sequence-id-483380

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  24. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  25. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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  28. RIB VP64 R1

    Plasmid
    #161493
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R1 in the RIB1 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161493-plasmid-map-sequence-id-326164

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  34. RIB VP64 R2

    Plasmid
    #161494
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R2 in the RIB1 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161494-plasmid-map-sequence-id-326165

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  35. RIB VP64 R3

    Plasmid
    #161495
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R3 in the RIB1 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161495-plasmid-map-sequence-id-326166

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  36. RIB VP64 R4

    Plasmid
    #161496
    Purpose
    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Targeting R4 in the RIB1 promoter)
    Expression
    Yeast
    Promoter
    pGAP, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161496-plasmid-map-sequence-id-326167

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  38. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  40. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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Showing: 1161 - 1200 of 2326 results