We narrowed to 22,678 results for: Sis;

1. pMBA323

   Plasmid

   #214751

   Purpose

   To create delta(glmS,nadE) working strains and maintaining their viability

   Depositor

   Tae Seok Moon

   Article

   Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

   Insert

   BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+/N-SNAPf

   Plasmid

   #184415

   Purpose

   vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Expression

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Promoter

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+/C-SNAPf

   Plasmid

   #184416

   Purpose

   vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Expression

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Promoter

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-Z11

   Plasmid

   #120054

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet-i
   

   Use

   Pcr template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA-G-intron-G

   Plasmid

   #40027

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP exon 1
   GFP exon 2
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of a loxP site, nucleotides 1-274, and …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available Since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-FLAG TIF1gamma M (456-887)

   Plasmid

   #15735

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   TIF1 gamma M (TRIM33 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Middle region (amino acids 456-887).

   Promoter

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKPY197

   Plasmid

   #62599

   Purpose

   Encodes Thr412Gly-CePheRS Alpha Subunit

   Depositor

   David Tirrell

   Article

   Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

   Insert

   Thr412Gly-CePheRS Alpha Subunit
   

   Use

   Tags

   HIS

   Expression

   Worm

   Mutation

   Thr412Gly

   Promoter

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrichoGate-4

   Plasmid

   #160704

   Purpose

   Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   optimised synthetic mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGENE:EFF-1::mCherry::V5

   Plasmid

   #133020

   Purpose

   EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

   Insert

   EFF-1::mCherry::V5
   

   Use

   Tags

   V5; mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXTm-zebrafish Tspan4a

    Plasmid

    #210327

    Purpose

    Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

    Depositor

    Li Yu

    Article

    Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

    Insert

    Tetraspanin 4a (tspan4a Zebrafish)
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Abp1-tDimerRFP

    Plasmid

    #27699

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Abp1 (Dbnl Mouse)
    

    Use

    Tags

    tDimer-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-mCherry2-G

    Plasmid

    #164162

    Purpose

    Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEarleyGate103 mmPing20F

    Plasmid

    #140007

    Purpose

    mmPing20F activation tag

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Insert

    mmPing20F
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMaCTag-P02

    Plasmid

    #120013

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TagBFP
    

    Use

    Pcr template

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR macroH2A1.2-C

    Plasmid

    #30512

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Gateway - donor vector

    Tags

    Expression

    Mutation

    NO STOP

    Promoter

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Grp1-PH pET11a

    Plasmid

    #71377

    Purpose

    Phosphoinositide Binding

    Depositor

    Mark Lemmon

    Article

    Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pEGFP-C1-hEFA6A

    Plasmid

    #206412

    Purpose

    Mammalian expression of hEFA6A

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6A (PSD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF1α-mouse-retroCHMP3-HA

    Plasmid

    #154178

    Purpose

    expresses mouse retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB6HindIII-rat FcRn

    Plasmid

    #121671

    Purpose

    Expresses rat neonatal Fc receptor in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat neonatal Fc receptor (Fcgrt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTH

    Plasmid

    #56659

    Purpose

    Dual-expression vector for P. tricornutum

    Depositor

    Philip Weyman

    Article

    Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

    Insert

    KanR
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCS2+ Bmi1-cherry-HA

    Plasmid

    #30516

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Xenopus/zebrafish expression

    Tags

    cherry and triple HA

    Expression

    Mammalian

    Mutation

    NO STOP

    Promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWZL blast HA goosecoid

    Plasmid

    #14096

    Depositor

    Bob Weinberg

    Article

    Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

    Insert

    goosecoid (Gsc Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pRU52

    Plasmid

    #167678

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-EF1a-NucGFP

    Plasmid

    #184696

    Purpose

    Lentiviral transfer plasmid for expression of NucGFP

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    NucGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPQD0CM0068

    Plasmid

    #177025

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    major latex protein-like
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAGM28521

    Plasmid

    #105359

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    EC1.2en-EC1.1p fusion promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCV-Pidd1(T788D)

    Plasmid

    #60530

    Purpose

    Retroviral expression of Pidd1 T788D phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Aspartic acid*

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pMIR-9

    Plasmid

    #25037

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 binding site (MIR9-1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only