We narrowed to 5,621 results for: pcas

1. pCAG-OSF-KATNA1

   Plasmid

   #160051

   Purpose

   Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   One-Strep-Flag (OSF)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GFP-Rab27a

   Plasmid

   #194985

   Purpose

   overexpress Rab27a

   Depositor

   Angelique Bordey

   Insert

   GFP-Rab27a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS9-mCherry-Frame +0

   Plasmid

   #66939

   Purpose

   CRISPaint frame selector +0

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9-mCherry-Frame +2

   Plasmid

   #66941

   Purpose

   CRISPaint frame selector +2

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +2
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-mCherry-Frame +1

   Plasmid

   #66940

   Purpose

   CRISPaint frame selector +1

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-G-Flamp2-mut

   Plasmid

   #192783

   Purpose

   control probe of G-Flamp2, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Liu et al Front Pharmacol. 2022 May 12;13:902290.

   Insert

   G-Flamp2-mut
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Sema4D-Flag

   Plasmid

   #190646

   Purpose

   Expresses Mouse Sema4D with C Terminal Flag Tag

   Depositor

   Victor Tarabykin, Andrew Newman

   Article

   Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

   Insert

   Sema4d (Sema4d Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-sp-myc-mSema4D

    Plasmid

    #190645

    Purpose

    Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-NLS-HA-Bxb1

    Plasmid

    #51271

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-loxPSTOPloxP-ZsGreen

    Plasmid

    #51269

    Purpose

    Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    loxP-STOP-loxP-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-MBP-ATG9

    Plasmid

    #188076

    Purpose

    Recombinant overexpression of human ATG9 in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG_Xph20-mRuby2-CCR5TC

    Plasmid

    #135531

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Use

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-HA-rat Nrxn1alpha

    Plasmid

    #58266

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1alpha

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1alpha (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-roxSTOProx-ZsGreen

    Plasmid

    #51274

    Purpose

    Dre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by Dre-recognition sites (rox) followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    roxSTOProx-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pX551-miniCMV-SpCas9

    Plasmid

    #107031

    Purpose

    AAV plasmid expressing Cas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCAG-WHaloCaMP1a-EGFP

    Plasmid

    #205313

    Purpose

    Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SpCas9-CBE6b-V106W

    Plasmid

    #215825

    Purpose

    Express CBE6b V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCI flag PCAF

    Plasmid

    #8941

    Depositor

    Yoshihiro Nakatani

    Article

    Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

    Insert

    PCAF (KAT2B Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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27. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAG-Cre-iBSD

    Plasmid

    #140284

    Purpose

    Expression of Cre-IRES-BSD

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG mito-RCaMP1h

    Plasmid

    #105013

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAX FLAG APP

    Plasmid

    #30154

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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