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pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
Beta-arrestin2 K11,12R GFP

Plasmid

#35412

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K11,12R

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

Plasmid

#179438

Purpose

Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-KRAB

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn1

Plasmid

#59339

Purpose

Lentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 1 shRNA (rat) (Nlgn1 Rat)
eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and mouse U6

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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BE3-NLS-NLS-P2A-BLAST

Plasmid

#135348

Purpose

Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

BE3-2XNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPbeta

Plasmid

#13393

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPbeta (Cebpb Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
CD200RCd4d3+4-blac

Plasmid

#32407

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Use

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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FH95pUp95GW (B4)

Plasmid

#74013

Purpose

lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

Depositor

Weifeng Xu, Robert Malenka, Oliver Schluter

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-rat PKA-RIIbeta-mEGFP

Plasmid

#45530

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Use

With chicken beta actin promoter and wpre

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-rat PKA-RIIbeta

Plasmid

#45522

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PKCZeta KR HA Tagged

Plasmid

#24608

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (Prkcz Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

K281R mutation in the ATP binding domain causing …

Promoter

Available since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Clover-Lysosomes-20

Plasmid

#56528

Purpose

Localization: Lysosome Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
RnBarr2-mTurquoise2

Plasmid

#137792

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-P2X2-CFP

Plasmid

#22401

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pRB144

Plasmid

#180175

Purpose

pCDNA3.1 Construct GluN1 A714L

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN1 (Grin1 Rat)

Use

Tags

Phluorin

Expression

Mammalian

Mutation

A714L

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-RSV-jGCaMP7f-W

Plasmid

#126691

Purpose

Mammalian calcium sensor protein.

Depositor

Darrell Kotton

Insert

jGCaMP7f

Use

Lentiviral

Tags

6xHIS, T7, Xpress

Expression

Mutation

H78K, A317L

Promoter

RSV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 AU1 mTOR S2035W

Plasmid

#15678

Depositor

John Lawrence

Article

McMahon et al Mol Cell Biol. 2002 Nov . 22(21):7428-38

Insert

mTOR (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

S2035W

Promoter

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

SpeI

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin 77-465

Plasmid

#45970

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 77-465 (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-HA-S6K1-F5A-E389

Plasmid

#8988

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCLC3sGFP

Plasmid

#52423

Purpose

mammalian expression of rat CLC3 short transcript fused to EGFP

Depositor

Steven Weinman

Article

Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

Insert

CLC3 (Clcn3 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-wtVHL

Plasmid

#13389

Depositor

Lucy Anderson

Article

Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

Insert

von Hippel-Lindau (Vhl Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
LXSN-Cx43

Plasmid

#60692

Purpose

retroviral expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pXCX.CMV.CREB.WPRE

Plasmid

#38050

Depositor

James Uney

Article

Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

Insert

CREB (Creb1 Rat)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-sEspin-C-18

Plasmid

#54253

Purpose

Localization: Actin Bundling, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

sEspin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-sEspin-N-18

Plasmid

#54254

Purpose

Localization: Actin Bundling, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

sEspin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSG5-FLAG-CaMKKbeta rat FL

Plasmid

#32449

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S3L, G96R, and P108L

Promoter

SV40

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
pLV-shGrm2-2344

Plasmid

#120725

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)

Depositor

Amy Lasek

Article

Ding et al Alcohol Clin Exp Res. 2017 Nov;41(11):18

Insert

Grm2 (Grm2 Rat)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-shGrm2-972

Plasmid

#120724

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)

Depositor

Amy Lasek

Article

Ding et al Alcohol Clin Exp Res. 2017 Nov;41(11):18

Insert

Grm2 (Grm2 Rat)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RnAT1aR-mCherry

Plasmid

#137782

Purpose

Visualization of At1aR

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat AT1a receptor (Agtr1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV(X) C/EBPalpha

Plasmid

#13392

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPalpha (Cebpa Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
7iBi89 (rat fibronectin)

Plasmid

#14065

Depositor

Richard Hynes

Article

Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

Insert

Rat Fibronectin 7iBi89 (Fn1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Insert was cut out of pGEM3 with SalI and SacI an…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBABE hygro rat IRS-3

Plasmid

#11450

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-3 (Irs3 Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
mApple-CathespinB-6

Plasmid

#54871

Purpose

Localization: Lysosomes, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

CathespinB

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pG/N795

Plasmid

#108220

Purpose

Yeast expression vector for rat glucocorticoid receptor

Depositor

Didier Picard, Keith Yamamoto

Article

Schena et al Methods Enzymol. 1991;194:389-98.

Insert

Rat glucocorticoid receptor

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Use

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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