We narrowed to 6,070 results for: nls

1. pICE-NLS-mCherry

   Plasmid

   #60364

   Purpose

   Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   NLS-mCherry
   

   Use

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mutation

   Promoter

   CMV-tet

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-2C-NLS in pCSDest

   Plasmid

   #126638

   Purpose

   Expresses LbCas12a-2C-NLS in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 459 p3E-2A-nlsEGFPpA

   Plasmid

   #195969

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-nlsEGFP (nuclear)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-dCas9-trCIB1

   Plasmid

   #64119

   Purpose

   Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   dCas9-trCIB1 fusion
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   dCas9 (D10A H840A, catalytically inactive), trCIB…

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHeACT-mEGFP-NLS

   Plasmid

   #205003

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAd-PV-NLS-DsRed

   Plasmid

   #17287

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-DsRed-NLS-CD (Pvalb Rat)
   

   Use

   Adenoviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCSIIbsr-EKARrEV-NLS

   Plasmid

   #173854

   Purpose

   A lentiviral vector for EKARrEV-NLS

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   EKARrEV-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. spCas9-NLS-MAV

   Plasmid

   #124212

   Purpose

   SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

   Depositor

   Uri David Akavia

   Article

   Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   His6, MBP, TEV Cleavage site, and two copies of S…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-TO-MCP-3XBFPnls

    Plasmid

    #75383

    Purpose

    MCP-3XBFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    MCP
    

    Use

    Lentiviral

    Tags

    3XBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2CG2-QUAS5x:GFPNLS-SV40pA

    Plasmid

    #155122

    Purpose

    Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUAS5x:GFPNLS
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    QUAS5x-E1b

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pN1-NLS-pHlu

    Plasmid

    #129320

    Purpose

    pH measurement, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    pHluorin (from H. Imamura)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GANES-DEVD-BNLS

    Plasmid

    #50842

    Purpose

    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    NES sequence LALKLAGLDIGS placed after ddGFP A an…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCS:GFPnls-T2a-ER-Cre-ER

    Plasmid

    #200106

    Purpose

    Vector for LFNG

    Depositor

    Prayag Murawala

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-NLS-QUE37C

    Plasmid

    #129323

    Purpose

    ATP measurement, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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