We narrowed to 6,177 results for: nls

1. mPlum-PCNA-19-SV40NLS-4

   Plasmid

   #55994

   Purpose

   Localization: Nucleus, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mPlum

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTetOne-tdTomato-cNLS

   Plasmid

   #138529

   Purpose

   Inducible expression of tdTomato with nuclear localization signal

   Depositor

   Ana Teixeira, Christopher Grigsby

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE3Gs

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-MCP-AP_pLX304

   Plasmid

   #120918

   Purpose

   Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   NLS-MCP-AP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-His:PYL1:nls:VPR

   Plasmid

   #135988

   Purpose

   Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   PYL1 domain fused to the VPR activation domain
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 766 p3E-2A-nlsmCherrypA

   Plasmid

   #195972

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-nlsmCherry (nuclear)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xNLS-pMJ915v2-sfGFP

   Plasmid

   #88921

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQC NLS-CFP IX

   Plasmid

   #37334

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   NLS CFP
   

   Promoter

   CMV

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. RANLS-DEVD-BNES

   Plasmid

   #50840

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBbsr2-EKARrEV-NLS

    Plasmid

    #173855

    Purpose

    Encoding EKARrEV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis-parallel1-NLSH2BCas9

    Plasmid

    #112065

    Purpose

    Expression and purification of NLSH2BCas9 for fungal gene editing

    Depositor

    Jeff Coleman

    Article

    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

    Insert

    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    

    Use

    CRISPR

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Cas9 was codon optimized for Aspergillus niger

    Promoter

    promoter in vector

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIB1(deltaNLS)-pmGFP

    Plasmid

    #28240

    Purpose

    Expresses CIB1 fused to plasma membrane-localized EGFP

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. GANLS-DEVD-BNES

    Plasmid

    #50835

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddRFP B
    

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-enCas12a-2xNLS

    Plasmid

    #209021

    Purpose

    Lentiviral vector expressing human codon-optimized enCas12a with 2xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    enCas12a-2xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R/K548R

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-NLS-mCherry

    Plasmid

    #60364

    Purpose

    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    NLS-mCherry
    

    Tags

    NLS from SV40 T antigen and mCherry

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-dCas9-trCIB1

    Plasmid

    #64119

    Purpose

    Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    dCas9-trCIB1 fusion
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    dCas9 (D10A H840A, catalytically inactive), trCIB…

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGG-TurboID-3XNLS

    Plasmid

    #209392

    Purpose

    Transiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-3XNLS
    

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUGW NLS-Cre

    Plasmid

    #183425

    Purpose

    Lentiviral Cre vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    NLS-Cre
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-Lb-Cas12a-2xNLS

    Plasmid

    #155046

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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