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We narrowed to 13,669 results for: Egf

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miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

Plasmid

#167820

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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PX458-sgEIF4EBP1

Plasmid

#128097

Purpose

CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT1-T777I

Plasmid

#116398

Purpose

Lentiviral expression of FLT1 T777I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

T777I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-puro

Plasmid

#167824

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-puro

Plasmid

#167830

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1401

Plasmid

#29198

Purpose

High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple67 (FEV Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Expression

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT4-F131S

Plasmid

#116401

Purpose

Lentiviral expression of FLT4 F131S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Tags

Expression

Mutation

F131S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

Plasmid

#167826

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_EYS_p.N269H

Plasmid

#81557

Purpose

Gateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EYS (EYS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N269H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG_G-PTEN

Plasmid

#227438

Purpose

An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor (Pten Rat)

Use

AAV

Tags

CD-sREACh and mEGFP

Expression

Mammalian

Mutation

R14G

Promoter

pCAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninV-

Plasmid

#229696

Purpose

Transient expression of GFP-alpha-cateninV- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L344P

Promoter

CMV promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FLT1-COMP5AP-AviTag-9xHis

Plasmid

#157526

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FLT1 (FLT1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FLT4-COMP5AP-AviTag-9xHis

Plasmid

#157535

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FLT4 (FLT4 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSLED.RAB

Plasmid

#193015

Purpose

Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

Depositor

Jonathan Ling

Article

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP

Plasmid

#180795

Purpose

Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAecto&tm-PD-1int (BTLA, PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 (optimized for N-terminal knock-in)

Plasmid

#172608

Purpose

Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4(TA)-ires-blast

Plasmid

#167831

Purpose

Control sensor for EKAREN4

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E7-D192D

Plasmid

#154024

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Mutation

BAP1 c.576C>T, p.D192D

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E11-G312G

Plasmid

#154026

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Mutation

BAP1 c.936T>G, p.G312G

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT1-G886V

Plasmid

#116394

Purpose

Lentiviral expression of FLT1 G886V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

G886V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT1-L778P

Plasmid

#116396

Purpose

Lentiviral expression of FLT1 L778P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

L778P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT1-R781Q

Plasmid

#116397

Purpose

Lentiviral expression of FLT1 R781Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

R781Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT1-V395I

Plasmid

#116399

Purpose

Lentiviral expression of FLT1 V395I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

V395I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FLT1-W589*

Plasmid

#116400

Purpose

Lentiviral expression of FLT1 W589*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT1 (FLT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

W589*

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_EYS_p.F336L

Plasmid

#81582

Purpose

Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EYS (EYS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F336L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_EYS_p.H466N

Plasmid

#81517

Purpose

Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EYS (EYS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

H466N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_EYS_p.R26Q

Plasmid

#81523

Purpose

Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EYS (EYS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R26Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_EYS_p.R587I

Plasmid

#81485

Purpose

Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EYS (EYS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R587I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge