19,658 results

1. pcDNA3.1 Flag hHMGB1

   Plasmid

   #31609

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB1 (HMGB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGJK_His-SUMO-LbuCas13a

   Plasmid

   #172488

   Purpose

   Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   bdSUMO-LbuCas13a (codon-optimized)
   

   Tags

   His6 and bdSUMO

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP381

   Plasmid

   #158798

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-2
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   RuvC-inactivating mutation D394A

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG CMV14 / BRAF-V600E

   Plasmid

   #131723

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   human ChAT promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-flag S127A YAP

   Plasmid

   #27370

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   S127A ( major phosphorylation site in YAP2)

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Puro-CAG-ASAP2s

    Plasmid

    #101274

    Purpose

    Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. SaKKH-ABE8e

    Plasmid

    #138502

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9 (KKH)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-VAMP8

    Plasmid

    #45912

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle-associated membrane protein 8 (VAMP8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Tags

    mTFP1

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1982

    Plasmid

    #49113

    Purpose

    AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

    Insert

    ssAAV-Ple261-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CLDN5

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Kate Fitzgerald, Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

16. AIMTOR T757

    Plasmid

    #140828

    Purpose

    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR T757 (ULK1 Human)
    

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIBN(deltaNLS)-pmGFP

    Plasmid

    #26867

    Purpose

    Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-tdTomato

    Plasmid

    #99116

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-tdTomato
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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