We narrowed to 11,618 results for: SHA

1. CRISPseq-BFP-backbone

   Plasmid

   #85707

   Purpose

   Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

   Depositor

   Ido Amit

   Article

   Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

   Plasmid

   #101297

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-906 of alpha-Catenin

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

   Plasmid

   #89150

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-ATN1

   Plasmid

   #16179

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   Atrophin 1 (ATN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pEF1α:flex-mcherry

   Plasmid

   #222618

   Purpose

   Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

   Depositor

   Franck Polleux

   Article

   Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

   Insert

   Cre-dependent mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLC-242

   Plasmid

   #73189

   Purpose

   Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   chloramphenicol promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. loSIIN

   Plasmid

   #185663

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LVDP-CArG-RE-GPR

   Plasmid

   #89762

   Purpose

   Quantitative measurement of CArG response element activity.

   Depositor

   Stelios Andreadis

   Article

   Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

   Insert

   6X CArG-RE
   

   Use

   Lentiviral

   Tags

   CMV-ZsGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-AAV-sh[SNCA]

   Plasmid

   #75437

   Purpose

   knockdown alpha-synuclein in rat neurons

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   snca (Snca Rat)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. noSIIN

    Plasmid

    #185666

    Purpose

    shApc-expressing lentiviruses with no expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMD19_STARR-seq_plants

    Plasmid

    #117379

    Purpose

    STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

    Depositor

    Xiaoyu Zhang

    Article

    Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    GFP derived from pMDC107

    Expression

    Plant

    Mutation

    Promoter

    35s core promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EFS-Vector

    Plasmid

    #109395

    Purpose

    AAV-CRISPR knockout plasmid for direct in vivo genome editing

    Depositor

    Sidi Chen

    Article

    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFs-NS

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_V5-NEUROD1

    Plasmid

    #215031

    Purpose

    Mammalian expression of human NEUROD1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    neuronal differentiation 1 (NEUROD1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. dTAG NLRP1

    Plasmid

    #166825

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

    Plasmid

    #100275

    Purpose

    AAV-CRISPR mediated genome editing in glia / astrocytes

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. TMEM16A-I546K-peGFP-N1

    Plasmid

    #228582

    Purpose

    Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I546K

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-hDKK1-flag

    Plasmid

    #15494

    Depositor

    Sergei Sokol

    Article

    Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

    Insert

    DKK1 (DKK1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

20. p23-NES-LwaCas13a-msfGFP-NES-Flag

    Plasmid

    #165070

    Purpose

    overexpression of LwaCas13a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    LwaCas13a
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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