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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-SMN

Plasmid

#37057

Depositor

Greg Matera

Article

Shpargel et al Proc Natl Acad Sci U S A. 2005 Nov 29;10

Insert

Human SMN1 (SMN1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pHCMV_YFV_PrME

Plasmid

#207315

Purpose

Mammalian expression of Yellow fever virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

YFV_PrME (POLY Yellow fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

Plasmid

#208697

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3h

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAKlux2

Plasmid

#14080

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Promoter

Lac, T3

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSMART-SOS-GFPuv

Plasmid

#102283

Purpose

Fluorescent sensor of E. coli SOS response

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

cda-GFPuv

Expression

Bacterial

Promoter

cda SOS promoter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

Plasmid

#248034

Purpose

Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged Neoleukin-2/15

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-mCherry-SKL-22Xm6A MS2

Plasmid

#235128

Purpose

mCherry reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Article

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-mCherry-SKL-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Human, Synthetic)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-ExRai-CKAR2

Plasmid

#236099

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to ER surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

CYP450-ExRai-CKAR2

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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LTB4R-DuET

Plasmid

#213333

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

LTB4R (LTB4R Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ E3_5 _hFc

Plasmid

#199617

Purpose

Encodes DARPin-hFc E3_5 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc E3_5 (control)

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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VEE-EGFP-IRES-PuroR

Plasmid

#242414

Purpose

Spectral unmixing: EGFP only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

EGFP
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGag-mCherry

Plasmid

#212631

Purpose

Expression vector for an MLV Gag-mCherry fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAmCyan1-C1-ADCY6

Plasmid

#113905

Purpose

N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 (ADCY6 Human)

Tags

Cyan

Expression

Bacterial and Mammalian

Promoter

pCMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-NUP98

Plasmid

#229594

Purpose

Expresses mBaoJin-NUP98 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDSG372

Plasmid

#115602

Purpose

surface-displayed Nb1; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-FOXM1-shRNA-81

Plasmid

#89495

Purpose

Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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