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We narrowed to 6,999 results for: crispr cas9 plasmids

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Showing: 621 - 640 of 6999 results

  4. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Mutation
    D10A and H839A point mutations
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  7. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  11. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

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  12. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  13. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201152
    Purpose
    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    dCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: TACGTTCTCTATCACTGAT
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201152-plasmid-map-sequence-id-401799

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  14. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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Showing: 621 - 640 of 6999 results