We narrowed to 6,905 results for: crispr cas9 plasmids

1. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-SaCas9-U6-sgSlc17a7

   Plasmid

   #124846

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BsaI)_CBh-Cas9

   Plasmid

   #135010

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3xFLAG (N terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

   Plasmid

   #124859

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Use

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-NL-DHFR-SpCas9

   Plasmid

   #124522

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NL-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-CAGGS-dCas9

   Plasmid

   #110823

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid

    #71684

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid

    #71685

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAct:dCas9-VPR

    Plasmid

    #78898

    Purpose

    Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJWV102-wtcas9sp

    Plasmid

    #99612

    Purpose

    Plasmid containing wild-type cas9sp

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    cas9sp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PczcD

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-dCas9VP192-T2A-EGFP

    Plasmid

    #69536

    Purpose

    Episomal plasmid encoding dCas9VP192

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-EGFP
    

    Use

    CRISPR

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE EF1α dCas9-NED

    Plasmid

    #109369

    Purpose

    Lentiviral vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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