We narrowed to 59,662 results for: plasmid
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Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorPublicationInsertCodon optimized Cas9
UseCRISPRTags3x FLAGMutationSilent change from C to A at bp 834 of insertAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
myc-Rictor
Plasmid#1860PurposePlease see Addgene item #11367 for an updated version of this plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRictor (RICTOR Human)
TagsMycExpressionMammalianAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL2680
Plasmid#85581PurposeReplicating base vector for constructing cpf1/CRISPR editing plasmidsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas serviceAAV8 and AAV RetrogradeInserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCXLE-hOCT3/4-shp53-F
Plasmid#27077PurposeIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53DepositorPublicationInsertOCT3/4 (POU5F1 Human)
ExpressionMammalianAvailable sinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-hM3D(Gq)-mCherry
Plasmid#50478PurposeGq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoterDepositorHas serviceAAV5InserthM3D(Gq)-mCherry
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phDLX1-N174
Plasmid#60859Purpose2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoterDepositorAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiCRISPRv2GFP
Plasmid#82416Purpose3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning siteDepositorInsertGFP
UseCRISPR and LentiviralPromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-Cre
Plasmid#55636PurposeExpresses Cre in Mammalian CellsDepositorHas serviceAAV11 and AAV RetrogradeInsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCXLE-hSK
Plasmid#27078PurposeIntegration-free (episomal) expression of human SOX2 and KLF4DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-U6-sgRNA-CMV-GFP
Plasmid#85451PurposeExpress sgRNA in mammalian cellsDepositorInsertpCMV-EGFP
UseAAVPromoterpCMV-EGFPAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV Puro
Plasmid#68469PurposeRetroviral plasmid with multiple cloning site and puromycin resistanceDepositorInsertPuromycin Resistance
UseRetroviralExpressionMammalianPromoterpGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1-FIRE-pHLy
Plasmid#170775PurposeRatiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterCMVAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by