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19,776 results

Showing: 9661 - 9690 of 19776 results

  4. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  7. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    Yeast ADH1 promoter
    Available Since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51056-plasmid-map-sequence-id-77326

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  16. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72268-plasmid-map-sequence-id-134573

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  22. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  23. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171385
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-RESCUE-S
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171385-plasmid-map-sequence-id-337353

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  29. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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Showing: 9661 - 9690 of 19776 results