We narrowed to 17,134 results for: Por

1. GFP-MuTMC2truncated

   Plasmid

   #154909

   Purpose

   For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-MuTMC2truncated
   

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-179 and 691-716

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL2

   Plasmid

   #184130

   Purpose

   For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Pmrp-1

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1

   Plasmid

   #184129

   Purpose

   For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

   Depositor

   Alejandro Aballay

   Article

   Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

   Insert

   MRP-1 (mrp-1 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   Pmrp-1 (3kb)

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTW080

   Plasmid

   #115928

   Purpose

   SGP insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 -98/30 SGP NA (No Aptamer)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGREAT17

   Plasmid

   #170905

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-SLC30A10-N43H

   Plasmid

   #82344

   Purpose

   Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N43H

   Promoter

   CMV

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PcDNA-DEST47-CypB-Del3 C-term GFP

   Plasmid

   #36136

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains amino acids 39-216 of cyclophilin B; G63…

   Promoter

   CMV

   Available Since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEF1a-T2-NLuc

   Plasmid

   #135938

   Purpose

   Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-SLC30A10-D248A

   Plasmid

   #82346

   Purpose

   Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D248A

   Promoter

   CMV

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. DYNLT1

    Plasmid

    #132528

    Purpose

    Human TCTEX-1, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLT1 (DYNLT1 Human)
    

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p.UTA.2.0-GL-2

    Plasmid

    #82440

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Mutation

    The complementary region for GL2 siRNA control wa…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPPC027.306

    Plasmid

    #171149

    Purpose

    Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIhh1.3K-Luc

    Plasmid

    #53604

    Purpose

    Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

    Depositor

    Xiangli Yang

    Article

    Wang et al Development. 2009 Dec;136(24):4143-53. d

    Insert

    Ihh promoter
    

    Use

    Luciferase; Reporter

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pMP472

    Plasmid

    #134997

    Purpose

    Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP498

    Plasmid

    #134998

    Purpose

    Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN021

    Plasmid

    #91667

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB1a2R+pNOS_Red-F_NOSt

    Plasmid

    #170888

    Purpose

    GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientation

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    p.S286Y Red mutant

    Promoter

    pNOS

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. TB:2xP53_RE:MiniP

    Plasmid

    #133881

    Purpose

    2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIneo-EloC

    Plasmid

    #31982

    Depositor

    Joan Conaway

    Article

    Brower et al J Biol Chem. 1999 May 7;274(19):13629-36

    Insert

    Elongin C (ELOC Human)
    

    Expression

    Mammalian

    Available Since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pPD3C

    Plasmid

    #86971

    Purpose

    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 fused to phosphite dehydrogenase and P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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