We narrowed to 4,416 results for: Pet2
-
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
TagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Human, Synthetic)
TagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB2-eGFP-6xHis
Plasmid#130982PurposeBacterial expression of mouse EB2-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
TagsStrepIIExpressionBacterialPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-Cerulean (526)
Plasmid#70716PurposeExpression vector for Cerulean purificationDepositorInsertCerulean
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LanCL2 (6xHis-LanCL2)
Plasmid#73407PurposeExpresses His tagged LanCL2DepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialPromoterT7Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_DmKHC[1-421]-SNAP-6xHis
Plasmid#196975PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tagDepositorAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT2
Plasmid#53558Purposebacterial expression of IFIT2DepositorAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-STING(pLxIS)
Plasmid#221333PurposeOverexpression of STING CTT in bacteriaDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop B
Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-dspB(E184Q)-6xHis
Plasmid#175802PurposePlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tagExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23-CBD-(-PP)-EGFP-H246,249D
Plasmid#12598DepositorTypeEmpty backboneTags6xHis and EGFPExpressionBacterialAvailable SinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Histag-Citrine (525)
Plasmid#70718PurposeExpression vector for Citrine purificationDepositorInsertCitrine
Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a SV40 ST full length
Plasmid#132636Purposeexpresses SV40 ST full length in E. coliDepositorInsertSV40 small t antigen
ExpressionBacterialAvailable SinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClfB_N2N3-HIS-ybbr
Plasmid#101717PurposeClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertClfB_N2N3
TagsH3V 3C, HIS, and ybbrExpressionBacterialPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp9 C287A-His
Plasmid#11830DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
pET28-H12TEV-hEIF5B-1~1220(FL)
Plasmid#208636Purposefor E. coli expression of human eIF5B (ENST00000289371)DepositorInserthuman eIF5B (ENST00000289371) (EIF5B Human)
Tags6xhisExpressionBacterialMutationContain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP3(307-574)
Plasmid#71481PurposeEncodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 3 (SENP3 Human)
TagsHexahistidineExpressionBacterialMutationDeleted amino acids 1-306 (catalytic domain only)PromoterT7Available SinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21b GFP-Rab10 Q68LHis
Plasmid#186015PurposeGFP-Rab10 Q68LHisDepositorInsertGFP-Rab10 Q68LHis (RAB10 Human)
ExpressionBacterialAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22-6His-SUMO-Nsp10
Plasmid#154960PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-APEX2
Plasmid#117751PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp7 C186A-His
Plasmid#11826DepositorAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP-YAP-N-IDR
Plasmid#166440PurposeBacterial expression of 6x His tagged mEGFP-YAP1-N-IDR fusion proteinDepositorInsertmEGFP, YAP-N-IDR(1-164) (YAP1 Human)
ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-160-GCN4
Plasmid#74608PurposeExpresses artificially dimerized Hook domain of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-160 (HOOK3 Human)
Tags6x His, GCN4, Strep II, and superfolder GFPExpressionBacterialAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
ExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-CLIP
Plasmid#188942PurposeT7 expression vector for making his6- and HA3-tagged CLIP proteinDepositorInserthis6-HA3-CLIP
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
TagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hisx6-Sirt6-H133Y
Plasmid#20279DepositorAvailable SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
Tags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-Strep-TEV-Nsp14
Plasmid#154955PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coliDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-His6-Ubc9-C93A
Plasmid#133910PurposeBacterial expression of His6-Ubc9-C93ADepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 93 to AlaninePromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(1-611)
Plasmid#39336DepositorInsertElongation factor 1 alpha-like protein
TagsHis-tagExpressionBacterialPromoterT7Available SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Gluc-EL222-his
Plasmid#172097PurposeExpresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteriaDepositorInsertGaussia Luciferase - EL222
ExpressionBacterialMutationGluc mutations - M43L, M110LPromoterT7Available SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+) LdtMt2 (131-408)
Plasmid#59337PurposeExpresses LdtMt2 for L,D-transpeptidationDepositorInsertLdtMt2 (131-408)
TagsHisExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop A
Plasmid#171776PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-SpyTag003 Loop A
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues V…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-myc-His
Plasmid#67864Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tagDepositorInsertMRPP3 (KIAA0391 Human)
TagsHis and mycExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only