We narrowed to 4,275 results for: Pet2

1. pET28a-Gluc-EL222-his

   Plasmid

   #172097

   Purpose

   Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - EL222
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gluc mutations - M43L, M110L

   Promoter

   T7

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28b(+)_S. cerevisiae Hbs1(1-611)

   Plasmid

   #39336

   Depositor

   SeWon Suh

   Insert

   Elongation factor 1 alpha-like protein
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET21a(+) LdtMt2 (131-408)

   Plasmid

   #59337

   Purpose

   Expresses LdtMt2 for L,D-transpeptidation

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   LdtMt2 (131-408)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET28-sfGFP-SpyTag003 Loop A

   Plasmid

   #171776

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-SpyTag003 Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   SpyTag003 and linkers inserted between residues V…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-MRPP3-myc-His

   Plasmid

   #67864

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   MRPP3 (KIAA0391 Human)
   

   Use

   Tags

   His and myc

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-MBP-(CBX1)3-eReader

   Plasmid

   #135090

   Purpose

   Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered x3
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-His6-Ubc9-C138A

   Plasmid

   #133911

   Purpose

   Bacterial expression of His6-Ubc9-C138A

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Cysteine 138 to Alanine

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-TAF4(827-909)

   Plasmid

   #105620

   Purpose

   bacterially express TAF4 HFD

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   TAF4-HFD(827-909) (Taf4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pet22b-mCherry-TEAD4-IDR

   Plasmid

   #166442

   Purpose

   Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mCherry, TEAD4-IDR (TEAD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET21a-mAIM2-deltaPYD-GST

    Plasmid

    #51534

    Purpose

    Express GST-tagged HIN domain of mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    GST and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET27b-ecDHFR-N23PP/S148A

    Plasmid

    #110829

    Purpose

    Bacterial expression of E.coli DHFR N23PP/S148A mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Hisx6-Sirt6-S56A

    Plasmid

    #20278

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Serine 56 to Alanine

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pET22-Strep-TEV-Nsp10

    Plasmid

    #154958

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Strep-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pET21a-hAIM2-PYD-GST

    Plasmid

    #51531

    Purpose

    Express GST-tagged PYD of hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GST and T7

    Expression

    Bacterial

    Mutation

    deletion of amino acids 102-343

    Promoter

    lac operator

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-NmCas9-Flag H588A

    Plasmid

    #109032

    Purpose

    H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET22b-EC12-129/138

    Plasmid

    #47926

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28 His-SUMO-CHMP2B

    Plasmid

    #232018

    Purpose

    Bacterial expression of His and SUMO tagged CHMP2B

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Use

    Tags

    6x His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-His6-UROD-N367A

    Plasmid

    #236740

    Purpose

    Protein overexpression of human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    Uroporphyrinogen decarboxylase human N367A (UROD Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-His6-GFP-UROD

    Plasmid

    #236741

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human (UROD Human, Synthetic)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a asyn(Δ103-130)

    Plasmid

    #242235

    Purpose

    Expresses alpha synuclein (missing exon 5, residues 103-130) in bacteria

    Depositor

    Jennifer Lee

    Article

    SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

    Insert

    asyn(Δ103-130)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    deletion of residues 103-130

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a asyn(66-140) E83A

    Plasmid

    #240583

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21a asyn(66-140) K80A

    Plasmid

    #240584

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K80A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21a asyn(66-140) K96A

    Plasmid

    #240585

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K96A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a asyn(66-140) K97A

    Plasmid

    #240586

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K97A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21a asyn(66-140) K102A

    Plasmid

    #240588

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K102A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21a asyn(66-140) D98A

    Plasmid

    #240587

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, D98A

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET23a-His-TEV-SpyCatcher3

    Plasmid

    #235149

    Purpose

    Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29_anti-SARS-CoV-2-NM1226

    Plasmid

    #209422

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1226
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pET29_anti-SARS-CoV-2-NM1230

    Plasmid

    #209423

    Purpose

    Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-NM1230
    

    Use

    Tags

    Myc-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pET29_anti-MERS-Nanobody-VHH-55

    Plasmid

    #209417

    Purpose

    Express anti-MERS-Nanobody-VHH-55 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-55
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pET29_anti-MERS-Nanobody-VHH-83

    Plasmid

    #209418

    Purpose

    express anti-MERS-Nanobody-VHH-83 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pET29_anti-MERS-Nanobody-VHH-84

    Plasmid

    #209419

    Purpose

    Express anti-MERS-Nanobody-VHH-84 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-83
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pET29_anti-SARS-CoV-2-Nanobody-C5

    Plasmid

    #209420

    Purpose

    Express anti-SARS-CoV-2-Nanobody C5 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-C5
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pET29_anti-SARS-CoV-2-Nanobody-F2

    Plasmid

    #209421

    Purpose

    Express anti-SARS-CoV-2-Nanobody F2 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-SARS-CoV-2-Nanobody-F2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pET29_anti-MERS-Nanobody-VHH-1

    Plasmid

    #206890

    Purpose

    Express anti-MERS-Nanobody-VHH-1 in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    anti-mers-nanobody-vhh-1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pET28b-SUMO-FLAG-TeKaiC

    Plasmid

    #229222

    Purpose

    For expression of T. elongatus KaiC protein

    Depositor

    Andy LiWang

    Insert

    kaiC (kaiC T. elongatus)
    

    Use

    Tags

    SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28b-SUMO-SeKaiB-FLAG

    Plasmid

    #229219

    Purpose

    For expression of S. elongatus KaiB protein

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB S. elongatus)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-hfYFP-TEV-Bcx

    Plasmid

    #191195

    Purpose

    Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Bcx fusion
    

    Use

    Tags

    6xHis and Bacillus circulans xylanase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28a-hfYFP-TEV-mScarlet-i

    Plasmid

    #191196

    Purpose

    Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-hfYFP-TEV-SAV

    Plasmid

    #191197

    Purpose

    Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28a-LSSmGFP-TEV-mScarlet-i

    Plasmid

    #191199

    Purpose

    Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & mScarlet-I fusion
    

    Use

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-LSSmGFP-TEV-SAV

    Plasmid

    #191200

    Purpose

    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & Streptavidin fusion
    

    Use

    Tags

    6xHis and Streptavidin core domain, amino acids 1…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET22b-T5-MfnG-TEV-His

    Plasmid

    #191114

    Purpose

    Expresses MfnG-TEV-His in E. coli

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET28.His.3C.86b.8D2Q.Tau2N4R

    Plasmid

    #226400

    Purpose

    Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau (MAPT Human, synthetic)
    

    Use

    Tags

    His.3C.86b

    Expression

    Bacterial

    Mutation

    8D2Q.Tau2N4R

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28.His.3C.86b.20D3Q.Tau2N4R

    Plasmid

    #226401

    Purpose

    Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

    Depositor

    Bradley Hyman

    Article

    Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

    Insert

    Tau2N4R (MAPT Human, synthetic)
    

    Use

    Tags

    His.3C.86b

    Expression

    Bacterial

    Mutation

    20D3Q, Tau2N4R

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET28-PKP3(305-797)

    Plasmid

    #202599

    Purpose

    Expresses ARM domain of plakophilin3

    Depositor

    Tina Izard

    Article

    Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

    Insert

    plakophilin3 (PKP3 Human)
    

    Use

    Tags

    His8 tag with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1 to 304

    Promoter

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge