We narrowed to 6,962 results for: tet on

1. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

   Plasmid

   #22032

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

   Plasmid

   #184380

   Purpose

   The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-TetON-mEGFP-HMGB1-MUT-Patchless

   Plasmid

   #194563

   Purpose

   Dox-inducible Mammalian Expression of mEGFP-HMGB1 Mutant "Patchless" (K184Rfs*44, "Patchless" variant) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   HMGB1 (HMGB1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   K184Rfs*44, truncation by stop codon after Lys209

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS-TarHom-Tol2-TetR-cryaa-YFP

   Plasmid

   #73202

   Purpose

   BAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker Citrine

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

   Plasmid

   #91873

   Purpose

   Photo-activatable/light sensitive Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   VAV2 DH/PH/ZF (Vav2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

   Plasmid

   #121799

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + CMV::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER

   Plasmid

   #52686

   Purpose

   Same as 41024, with additional linker

   Depositor

   Michael Nick Boddy

   Insert

   tetO7-Pcyc1-3xHA+LINKER
   

   Use

   Promoter replacement

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-PGK-FKBP-Ztetv-Cit-tvhc-DHFR

   Plasmid

   #116052

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   FKBP-Ztetv-Cit-tvhc-DHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEST-p15a-PLtetO-1-UTR1-LacI-T500

   Plasmid

   #45784

   Depositor

   Vincent Noireaux, Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   Lac repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtetO-1

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129036

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129029

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. GB1-His-TET1-878-911(pL91)

    Plasmid

    #124390

    Purpose

    E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911 wildtype (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEST-PLlacO-1-UTR1-TetR-YbaQ-T500

    Plasmid

    #45778

    Depositor

    Richard Murray

    Insert

    Tetracycline repressor
    

    Use

    Synthetic Biology

    Tags

    YbaQ degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    PLlacO-1

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

    Plasmid

    #108482

    Purpose

    inducible expression of vsv-Mis12-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    Mis12-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by Mis12

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEST-PLtetO-1-UTR1-LacI-YbaQ-T500

    Plasmid

    #45776

    Depositor

    Vincent Noireaux, Richard Murray

    Insert

    Lac repressor
    

    Use

    Synthetic Biology

    Tags

    YbaQ degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    PLtetO-1

    Available since

    Aug. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-kanMX-tetO-Pcyc1-GST-LINKER

    Plasmid

    #52688

    Purpose

    Same as 41026, with additional linker

    Depositor

    Michael Nick Boddy

    Insert

    tetO7-Pcyc1-GST+LINKER
    

    Use

    Promoter replacement

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXTK-BB4 [Backbone_repABC-pMlb-KanR-TetR_PartTypeBB]

    Plasmid

    #229390

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

    Plasmid

    #231601

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-TetO-Chim-Pchi-Q61A/E64M

    Plasmid

    #231521

    Purpose

    Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

    Depositor

    Ralf Jauch

    Article

    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

    Insert

    Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Point mutations at position 61 Q to A and 64 E to…

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225645

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225648

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225647

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    LacO3O1 and tetA promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225646

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tetA and LacO3O1 promoters

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Use

    Tags

    HA signal FALG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-flag-Lag16-fibcon-iCAM1-tether

    Plasmid

    #205213

    Purpose

    This vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRetroX-TetOne-Puro-p62 K7A/D69A

    Plasmid

    #204549

    Purpose

    Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K7A/D69A

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196081

    Purpose

    Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196083

    Purpose

    Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Tet-Inducible Mouse C325A Caspase-9 Mutant

    Plasmid

    #194500

    Purpose

    Inducible Expression of Mouse C325A Caspase-9 Mutant

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    Mouse Caspase-9 C325A Mutant (Casp9 Mouse)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Tet-inducible 3xFlag Mouse Caspase-9 ΔLS

    Plasmid

    #194609

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse Caspase-9 Minus Large Subunit Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse Caspase-9 Minus Sequence Encoding Val 177 to Asp 353 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Deletion of Large Subunit Domain eliminates bindi…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔA LS

    Plasmid

    #194926

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Large Subunit Region Val 177 to Arg 263

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Val 177 to Arg 263 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to Eliminate Prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193314

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Use

    Tags

    4MYC

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193315

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Use

    Tags

    4FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid

    #193316

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4MYC N-terminal tagging
    

    Use

    Tags

    4MYC

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G

    Plasmid

    #157982

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9

    Plasmid

    #129061

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA9 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-10

    Plasmid

    #129062

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA10 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-11

    Plasmid

    #129063

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA11 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-12

    Plasmid

    #129064

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA12 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-2

    Plasmid

    #129054

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA2 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-3

    Plasmid

    #129055

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA3 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-4

    Plasmid

    #129056

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA4 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-5

    Plasmid

    #129057

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA5 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge