We narrowed to 987 results for: Streptococcus

1. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

   Plasmid

   #165445

   Purpose

   Expresses ACX, rPB(8kD) in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rPB(8kD)
   

   Expression

   Mammalian

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG335

   Plasmid

   #100899

   Purpose

   SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGT6471

   Plasmid

   #219428

   Purpose

   Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
   

   Use

   Moclo compatible level 0 module

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA032

   Plasmid

   #216012

   Purpose

   Fragmid fragment: (Cas protein) deactivated Cas

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dCas9_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7-hSt3Cas9 (pXK-1721)

   Plasmid

   #208828

   Purpose

   CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.

   Depositor

   Kun Xu

   Article

   Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

   Type

   Empty backbone

   Use

   CRISPR; Stcas9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKMW306_U6-sgRNA-EMX1

   Plasmid

   #244824

   Purpose

   Mammalian expression of SpyCas9 single guide RNA targeting EMX1

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKMW124_dCas9

   Plasmid

   #244821

   Purpose

   Mammalian expression of catalytically inactive Cas9 (dCas9)

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   dCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHS26_10xHis_MBP_TEV_VRER-Cas9

    Plasmid

    #244829

    Purpose

    Bacterial expression of VRER Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    VRER Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135V/G1218R/R1335E/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS16_10xHis_MBP_TEV_xPBA-Cas9

    Plasmid

    #244830

    Purpose

    Bacterial expression of xPBA Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    xPBA Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS1_10xHis_MBP_TEV_WT-Cas9

    Plasmid

    #244825

    Purpose

    Bacterial expression of SpyCas9 (WT) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS25_10xHis_MBP_TEV_EQR-Cas9

    Plasmid

    #244828

    Purpose

    Bacterial expression of EQR Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    EQR Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135E/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS9_10xHis_MBP_TEV_dCas9

    Plasmid

    #244831

    Purpose

    Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dCas9 (WT)
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/H840A

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_734

    Plasmid

    #216097

    Purpose

    All-in-one, knockout, delivers Cas9 & gRNA

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSSA-DsRed/eGFP (pXK-442)

    Plasmid

    #208827

    Purpose

    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSSA-eGFP (pXK-221)

    Plasmid

    #208826

    Purpose

    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).

    Depositor

    Kun Xu

    Article

    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid

    #179526

    Purpose

    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 KES(1107-1109)GG
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    replaced KES (residues 1107-1109) with GG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid

    #179527

    Purpose

    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 K233A/K234A/K253A/K263A
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed lysines 233, 234, 253, and 263 to alanine

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT175_GalL_A3A(Y130F)Δ186-BE3

    Plasmid

    #145124

    Purpose

    Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)Δ186-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(Y130F; 1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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