We narrowed to 63,598 results for: cell

1. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xCBX7CD_W35A-IFP2.0C

   Plasmid

   #215755

   Purpose

   Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2xCBX7CD_W35A-IFP2.0C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRcCMV myc-Plk1 wt (Nigg RG6)

   Plasmid

   #41160

   Depositor

   Erich Nigg

   Article

   Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

   Insert

   Plk1 (PLK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-JNKKTRmRuby2

   Plasmid

   #59148

   Purpose

   ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. ETS1_pet28a

   Plasmid

   #131663

   Purpose

   Bacterial expression of ETS gene ETS1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETS1 (ETS1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

   Plasmid

   #154826

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   mCherry
   NeoR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215328

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   FOXJ1 Promoter
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FOXJ1

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5HT6-YFP-PIPK(D253A)

   Plasmid

   #96811

   Purpose

   kinase-dead control for 5HT6-YFP-PIPK

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-PIPK(D253A)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D253A inactivates kinase activity of PIPK

   Promoter

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA-mKeima-PIM

   Plasmid

   #174650

   Purpose

   Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

   Depositor

   Lukas Kapitein

   Article

   Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

   Insert

   xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

   Promoter

   Chicken beta-actin

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid

    #191154

    Purpose

    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP-t70587
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

    Plasmid

    #62956

    Purpose

    Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-Cremyc-2A-GFP

    Plasmid

    #116879

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBR_attB(C31)_FRT

    Plasmid

    #183761

    Purpose

    phiC31-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-PPP2R2B

    Plasmid

    #16181

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    PP2A regulatory subunit B, beta isoform (PPP2R2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

15. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLUT7 HA-GLI1

    Plasmid

    #62970

    Purpose

    Expresses HA-tagged Gli1

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5 DN-Fyn

    Plasmid

    #16033

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative: K299M

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

    Plasmid

    #69534

    Purpose

    DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    DD-dCas9VP192-T2A-EGFP
    

    Use

    Tags

    C-term T2A-EGFP and DHFR Destabilised Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Use

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

    Plasmid

    #62954

    Purpose

    Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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