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We narrowed to 20,246 results for: Sele

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Showing: 9901 - 9930 of 20246 results

  1. Flag-M4-AR-W741C

    Plasmid
    #171247
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys (AR-W741C)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171247-plasmid-map-sequence-id-336010

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  3. pcDNA3-sACE2v3-sfGFP

    Plasmid
    #145174
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145174-plasmid-map-sequence-id-292600

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  4. pGL3-Basic-Rab18-sfGFP(N) HDR template

    Plasmid
    #129415
    Purpose
    HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP
    Depositor
    Elina Ikonen
    Publication
    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB18 HDR template (RAB18 Human)
    Use
    CRISPR and TALEN; Endogenous tagging hdr template
    Tags
    sfGFP
    Expression
    Mammalian
    Available since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129415-plasmid-map-sequence-id-254821

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  6. pCIBN-tagBFP-TetR

    Plasmid
    #103797
    Purpose
    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Publication
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIBN-tagBFP-TetR (CIB1, tetR Mustard Weed, E. coli)
    Expression
    Mammalian
    Mutation
    TetR: A4G (M2V)
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103797-plasmid-map-sequence-id-199187

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  7. ET8-GPIba-6His

    Plasmid
    #102878
    Purpose
    Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus
    Depositor
    Timothy Springer
    Publication
    Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)
    Tags
    6xHis tag
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102878-plasmid-map-sequence-id-198799

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  13. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRM1 (GRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

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  14. pBA-mKeima-PIM

    Plasmid
    #174650
    Purpose
    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
    Depositor
    Lukas Kapitein
    Publication
    Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    Chicken beta-actin
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    174650-plasmid-map-sequence-id-346673

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  15. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Publication
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  16. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Publication
    Zahradník et al bioRxiv Preprint 2020
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162457-plasmid-map-sequence-id-321472

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  17. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79887-plasmid-map-sequence-id-402553

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  20. pEBTet-SNAP-Centrobin

    Plasmid
    #136829
    Purpose
    Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag
    Depositor
    Kai Johnsson
    Publication
    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-Centrobin (CNTROB Synthetic, Human)
    Tags
    FLAG-tag / His-tag
    Expression
    Mammalian
    Promoter
    CMV-TetO2
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    136829-plasmid-map-sequence-id-276002

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  21. pCEP4-myc-HLA-B8

    Plasmid
    #135512
    Purpose
    Mammalian expression of myc-tagged HLA-B*08:01
    Depositor
    Erik Procko
    Publication
    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HLA-B*08:01 (HLA-B Human)
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135512-plasmid-map-sequence-id-262684

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  22. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Publication
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  24. AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

    Plasmid
    #205443
    Purpose
    human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55
    Depositor
    Deepta Bhattacharya
    Publication
    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCD46-HLA-G SCT-hCD47-hCD59
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205443-plasmid-map-sequence-id-408942

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  25. pPHR-mCherry-hHP1gamma

    Plasmid
    #181885
    Purpose
    Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PHR-mCherry-hHP1gamma (CBX3 Mustard Weed, Human)
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181885-plasmid-map-sequence-id-359703

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  26. R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

    Plasmid
    #170201
    Purpose
    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)
    Depositor
    Dominic Esposito
    Publication
    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV51
    Available since
    Feb. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    170201-plasmid-map-sequence-id-334264

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  27. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid
    #131249
    Purpose
    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag
    Depositor
    Roger Woodgate
    Publication
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131249-plasmid-map-sequence-id-267665

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  28. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid
    #136451
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glycoprotein O (UL74 Human betaherpesvirus 5)
    Tags
    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136451-plasmid-map-sequence-id-271690

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  29. pcDNA3-ΔN-RNMT

    Plasmid
    #112708
    Purpose
    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.
    Depositor
    Daniel Schoenberg
    Publication
    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNMT (RNMT Human)
    Tags
    FLAG and HIV Rev nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    deleted amino acids 2-120, mutated active-site D2…
    Promoter
    CMV
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112708-plasmid-map-sequence-id-217040

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Showing: 9901 - 9930 of 20246 results