14,895 results

1. pDIV272

   Plasmid

   #226183

   Purpose

   Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCrispr-V2-mOrange (V2mO)

   Plasmid

   #140206

   Purpose

   Lenti Crispr/Cas9 with mOrange fluorescent marker

   Depositor

   Jaffer Ajani

   Article

   Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pELF1a

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWN_U6-B2M-miniGag-Cas9

   Plasmid

   #228958

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDT-sgRNA

   Plasmid

   #138271

   Purpose

   Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

   Insert

   sgBG, sgTS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-dCas9-VPR-pgk-hph

   Plasmid

   #233066

   Purpose

   Piggybac CRISPRa construct

   Depositor

   Markus Landthaler

   Article

   Vučićević et al bioRxiv 2024.09.12.612198

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-AAVS1_sgRNA

   Plasmid

   #183891

   Purpose

   pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-Dual-Guide-Donor-ShuttleOnly

   Plasmid

   #171098

   Purpose

   Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   XbaI site added upstream of U6 promotor. GtoC mu…

   Promoter

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ141D-peg

    Plasmid

    #141081

    Purpose

    Gateway compatible prime editing gRNA empty backbone vector

    Depositor

    Yiping Qi

    Article

    Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFETCh_KLF9

    Plasmid

    #86286

    Purpose

    Donor vector for 3' FLAG tag of human KLF9

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KLF9 homology arms (KLF9 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_KLF9_2

    Plasmid

    #86347

    Purpose

    Encodes gRNA for 3' target of human KLF9

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF9 (KLF9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_KLF9_1

    Plasmid

    #86346

    Purpose

    Encodes gRNA for 3' target of human KLF9

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF9 (KLF9 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6a:sgRNA#1

    Plasmid

    #64245

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

    Plasmid

    #244838

    Purpose

    Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

    Depositor

    Jennifer Doudna

    Article

    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

    Insert

    Flex-Cas12a
    

    Use

    CRISPR

    Tags

    2xSV40 NLS

    Expression

    Mammalian

    Mutation

    G146R/R182V/D535G/S551F/D665N/E795Q

    Promoter

    T7

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti NG-ABE8e_P2A_GFP + PuroR

    Plasmid

    #242000

    Purpose

    Lentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.

    Depositor

    Frank Buchholz

    Article

    Wang et al Genome Biol. 2025 Jul 22;26(1):217. doi:

    Insert

    NG-ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFD116

    Plasmid

    #124769

    Purpose

    pFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    dcas9
    PpflB sgRNA BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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