We narrowed to 7,333 results for: ef1a
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Plasmid#113158PurposeAn AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporterDepositorInsertgRNA for rat TH
UseAAV, CRISPR, and Cre/LoxExpressionMammalianPromotermU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Rad23b UbL
Plasmid#184703PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbLDepositorInsertGFP ArgSen delta Rad23b UbL
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9
Plasmid#163644PurposeMakes a lentivirus that expresses mNeonGreen and Cas9.DepositorInsertmNeonGreen-P2A-Cas9
UseLentiviralTagsmNeonGreen-P2APromoterEFSAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138747PurposeIntegration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertCAG
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
Plasmid#157951PurposeLentiviral expression of HBG site 1 sgRNADepositorInsertLenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138739PurposeIntegration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x0
UseSynthetic BiologyExpressionMammalianAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE
Plasmid#118279PurposeExpresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cellsDepositorInsertsmRuby2
ChR2(H134R)
UseAAV and Cre/LoxTagsSV40 NLS and YFPPromoterEF1 alphaAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)
Plasmid#117139PurposeExpression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFPDepositorInsertHA-Dmap1 (mutagenised) (Dmap1 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationmodified BFP, modified Dmap1 CDSPromoterhuman U6 promoter, human EF1a promoterAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75243PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75234PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75235PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75242PurposeCRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)DepositorInsertsgRNA against human Ikaros (IKZF1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75238PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc1 (1/2)DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)
Plasmid#75239PurposeCRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti
Plasmid#239610PurposeConstitutive active CRISPRgenee construct with a blasticidin resistanceDepositorInsertZIM3 KRAB domain (ZIM3 )
UseCRISPR and LentiviralAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo
Plasmid#239608PurposeBackbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistanceDepositorInsertfiller-trcr-H1-filler
UseCRISPR and LentiviralAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV EF1alpha INTS4 K26/27Q IRES Cherry
Plasmid#228112PurposeLentiviral expression of human INTS4 K26/27QDepositorInsertINTS4 (INTS4 Human)
UseLentiviralExpressionMammalianMutationMutated Lysine 26 and 27 to GlutaminePromoterEF 1alphaAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-TRIP12-391-938-EGFP-Hygro
Plasmid#238092PurposeExpression of structurally predicted WWE domain of TRIP12 with EGFP on C terminalDepositorInsertTRIP12 (TRIP12 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 319-938 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-TRIP12(749-836)-EGFP
Plasmid#196229PurposeEGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged TRIP12(749- 836)
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP
Plasmid#196235PurposeEGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged Af1521(K35E/Y145R)
UseLentiviralTagsEGFPExpressionMammalianMutationK35E and Y145RPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RNF146(100-182)-myc
Plasmid#196236PurposeRNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertRNF146(100-182) encoding a C-terminal myc tag
UseLentiviralTagsmyc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc
Plasmid#196237PurposeAf1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertAf1521(K35E/Y145R) encoding a C-terminal myc tag
UseLentiviralTagsmyc tagExpressionMammalianMutationamino acid 35 lysine is replaced with glutamic ac…PromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
(942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop
Plasmid#195624PurposepAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi constructDepositorInsertdCasphi
UseAAVTagsC-Myc (NLS), HA tag, and ZIM3ExpressionMammalianMutationP749A and P753APromoterEF1a (Mlu1/EcoR1)Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235301PurposeComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL(EF1a-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
Plasmid#236279PurposeLentiviral vector that can express hAqp1 with fkbp12 degron in mammalian cells. EF1a promoter. EGFP marker.DepositorInserthuman aquaporin 1 (AQP1 Human)
UseLentiviralTagsFlag and fkbp12ExpressionMammalianPromoterEF1aAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235302PurposeComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE
Plasmid#237446PurposeIntersectional expression of Myc-tagged synaptophysin in the presence of Cre and FlpDepositorInsertCon/Fon-Synaptophysin-10xMyc
UseAAVExpressionMammalianPromoterEF1aAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
Plasmid#233442PurposePiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)DepositorInsertFKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
ExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export
Plasmid#233381PurposeOptimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR2(ecto+TM)-tdMCP
TagsHAExpressionMammalianMutationWTPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA
Plasmid#233040PurposeTo express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequenceDepositorInsertmouse homeostatic iron regulator with H67D mutation (Hfe Mouse)
UseAAVTagsGFPMutationH67DAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa
Plasmid#233032PurposeTo Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genomeDepositorInsertCasRx and mCherry
UseAAVTagsmCherryAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2
Plasmid#231208PurposeSingle transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertmRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)
UseLentiviralMutationL80F, R81D, L85V, I86V, I92FAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2
Plasmid#231207PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertPuro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
UseLentiviralMutationL147I, R148H, I203V, L290VAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed
Plasmid#226590PurposePharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.DepositorInsertPSAML-141F,Y115F-GlyR
UseAAVTagsP2A-Fusion RedExpressionMammalianPromoterEF1aAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
Plasmid#199582PurposeExpresses Chronos-eGFP in a Cre-dependent mannerDepositorInsertChronos eGFP
UseAAV, CRISPR, and Cre/LoxTagsGFPExpressionMammalianPromoterEF1a intron AAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs
Plasmid#195018PurposeAn adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1DepositorInsertsCCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG
UseAAVTagsKASH domain of Nesprin2PromoterEF1a, hU6, and mU6Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G
Plasmid#204214PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationDeletion of DNA binding domain (delDBD)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G
Plasmid#204191PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationDeletion of R162PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G
Plasmid#204213PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on I207MPromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G
Plasmid#204211PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on Q175* (*termination)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G
Plasmid#204210PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralExpressionMammalianMutationPoint mutation on R178* (*termination)PromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G
Plasmid#204212PurposeDoxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpressionDepositorInsertNK2 homeobox 1 (NKX2-1 Human)
UseLentiviralTagsPoint mutation on I207FExpressionMammalianPromoterTetONAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen delta Degradation Domain
Plasmid#184692PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Degradation DomainDepositorInsertGFP ArgSen delta Degradation Domain
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen delta Disordered Region
Plasmid#184693PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Disordered RegionDepositorInsertGFP ArgSen delta Disordered Region
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen delta Rad23b UbL
Plasmid#184694PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbLDepositorInsertGFP ArgSen delta Rad23b UbL
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Degradation Domain
Plasmid#184701PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Degradation DomainDepositorInsertGFP ArgSen delta Degradation Domain
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Disordered Region
Plasmid#184702PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Disordered RegionDepositorInsertGFP ArgSen delta Disordered Region
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only