59,544 results

1. B-GRAM-W

   Plasmid

   #211707

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-EZH2

   Plasmid

   #28060

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF2102

   Plasmid

   #141923

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3/HA-FLAG-RBP2

   Plasmid

   #14800

   Depositor

   William Kaelin

   Article

   Klose et al Cell. 2007 Mar 9. 128(5):889-900.

   Insert

   Retinoblastoma Binding Protein 2 (KDM5A Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-sACE2-WT(732)-IgG1

   Plasmid

   #154104

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available Since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Tags

   AID and EYFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-HA-PSMD14

   Plasmid

   #22557

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   PSMD14 (PSMD14 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-28c(+)-YTHDF2

   Plasmid

   #102275

   Purpose

   For bacterial expression of His-tagged human YTHDF2

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Tags

   6X HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSimple-miR302/367

   Plasmid

   #98748

   Purpose

   Episomal reprogramming

   Depositor

   Sara Howden, James Thomson

   Article

   Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

   Insert

   miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABE-puro LEF1

    Plasmid

    #27023

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Tags

    V5 His

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV HDM2(C464A)

    Plasmid

    #12086

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Mutation

    C464A: RING-finger-domain mutant

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pET30-HPF1-His-Sumo-Flag

    Plasmid

    #111577

    Purpose

    Expresses full length HPF1 in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    HPF1 (HPF1 Human)
    

    Tags

    6xHis-Sumo-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-ArchT-BFP2-TSERex

    Plasmid

    #123312

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    ArchT-BFP2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BRD4

    Plasmid

    #38943

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CDK12 gRNA (BRDN0001147156)

    Plasmid

    #75915

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF0952

    Plasmid

    #143894

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223-DCAMKL2

    Plasmid

    #23604

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    DCAMKL2 (DCLK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pABM-D4-miR30-CypA

    Plasmid

    #132934

    Purpose

    Lentivector for CypA knockdown and blasticidin resistance

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    CypA shRNA
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Desmoglein2-mCherry

    Plasmid

    #36991

    Depositor

    Kathleen Green

    Article

    Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

    Insert

    Dsg2 (DSG2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge