We narrowed to 7,668 results for: CCH

1. pDSM-de-Ppxr1-PYC-Tnat1

   Plasmid

   #127736

   Purpose

   Yeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Ppxr1

   Availability

   Academic Institutions and Nonprofits only

   

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2. p414GPD-3xFLAG-Nop4 L306P

   Plasmid

   #169262

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 L306P (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   p.(Leu306Pro)

   Promoter

   GPD

   Availability

   Academic Institutions and Nonprofits only

   

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3. p414GPD-3xFLAG-Nop4 dE5

   Plasmid

   #169263

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E5 (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 5

   Promoter

   GPD

   Availability

   Academic Institutions and Nonprofits only

   

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4. p414GPD-3xFLAG-Nop4 dE8

   Plasmid

   #169264

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E8 (NOP4 Budding Yeast)
   

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 8

   Promoter

   GPD

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2GFP

   Plasmid

   #82416

   Purpose

   3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

   Depositor

   David Feldser

   Article

   Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

   Insert

   GFP
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS (P2A)

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisCoTune

   Plasmid

   #173713

   Purpose

   Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

   Depositor

   Morten Norholm

   Article

   Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

   Inserts

   ERV1 (ERV1 Budding Yeast)
   PDI
   

   Expression

   Bacterial

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAD100-CHIP-myc-His

   Plasmid

   #236054

   Purpose

   Mammalian expression of human CHIP with a C-terminal myc-His tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   CHIP (STUB1 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available Since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pNESx2-EGFP-C1-PABDx2-Spo20

   Plasmid

   #220082

   Purpose

   PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Tags

   X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

   Expression

   Mammalian

   Mutation

   amino acids 51-91, tandem dimer

   Promoter

   CMV

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-4591NES

    Plasmid

    #138377

    Purpose

    Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-ROCK
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN_HA-TBK1 (kinase dead - D135N)

    Plasmid

    #199206

    Purpose

    Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D135N

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184741

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

    Plasmid

    #184742

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCWXPGR-pTF-betaCatenin

    Plasmid

    #114281

    Purpose

    TET-inducible expression of a constitutively active form of the BetaCatenin protein

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    Catenin beta 1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    deletion of AA 1 to 45

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNatA (pACYCduet-naa10-naa15)

    Plasmid

    #72928

    Purpose

    Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Inserts

    naa10 (naa10 Fission Yeast, Synthetic)
    naa15 (naa15 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Codon optomised for E.coli expression

    Promoter

    T7

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN mEGFP-OPTN

    Plasmid

    #188784

    Purpose

    For mammalian expression of OPTN tagged with mEGFP

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-M13-TevC-IRES-SP6-Cre

    Plasmid

    #171620

    Purpose

    M13-TevC with Cre

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    M13-TevC-IRES-SP6-Cre
    

    Use

    AAV and Synthetic Biology

    Tags

    6x His

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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