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pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

Plasmid

#134869

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome b5 type A (CYB5A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-98

Promoter

EF1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-eLACCO2.1-COBRA

Plasmid

#223259

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-eLACCO2.1

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-eLACCO2-COBRA

Plasmid

#223258

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-eLACCO2

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgK-R-deLACCOctrl-COBRA

Plasmid

#223260

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-deLACCOctrl

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA C12 ecDHFR DD YFP HA

Plasmid

#192815

Purpose

Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

Depositor

John Hulleman

Article

Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

Insert

E. coli dihydrofolate reductase

Tags

enhanced yellow fluorescent protein and hemagglut…

Expression

Mammalian

Mutation

W74R/T113S/E120D/Q146L

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-EGFP-T2A-Bsd

Plasmid

#193198

Purpose

Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244102

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9L

Plasmid

#166788

Purpose

Insect cell expression vector for DPP9L (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Promoter

polyhedrin

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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