We narrowed to 20,464 results for: Cre

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LV_EF1a_CDK4-P2A-Hygro_Barcode

Plasmid

#170223

Purpose

Barcoded lentiviral vector to express CDK4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-IL6

Plasmid

#111933

Purpose

Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Interleukin 6 (IL6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-Fc-His

Plasmid

#72098

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMV361_MjTyrRS

Plasmid

#193250

Purpose

Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Insert

MjTyrRS

Expression

Bacterial

Promoter

hsp60

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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hyeA3A-BE4max

Plasmid

#157944

Purpose

Mammalian expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyeA3A-BE4max

Use

CRISPR

Expression

Mammalian

Mutation

N57G

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pX601-mCherry

Plasmid

#84039

Purpose

Staphylococcus aureus (SaCas9) conjugated with mCherry

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-WT-HA

Plasmid

#139845

Purpose

Lentiviral expression of human DDRGK1-WT-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-EYA1

Plasmid

#207209

Purpose

doxycycline-inducible expression of Human EYA1 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-EYA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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PTGDR-DuET

Plasmid

#213369

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

PTGDR (PTGDR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-WT-sgNT

Plasmid

#222120

Purpose

EIF3D overexpression vector with non-targeting sgRNA

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Control-Control-LRG-GFP

Plasmid

#225873

Purpose

Two copies of guide RNA targeting a control sequence

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

Control

Use

CRISPR and Lentiviral

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV mKeima-LC3

Plasmid

#189006

Purpose

Fluorescent reporter for autophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

mKeima, LC3 (Map1lc3b Rat)

Use

Retroviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-HP1alpha

Plasmid

#179701

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

Plasmid

#194279

Purpose

Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized VP64 dSaCas9 VP64 T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαq-LgB97

Plasmid

#134360

Purpose

Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαq-LgB97 (GNAQ Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOTL1

Plasmid

#172998

Purpose

constitutive expression of V5-tagged AMOTL1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOTL1 (AMOTL1 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 IRES Cherry

Plasmid

#232109

Purpose

Lentiviral expression of human INTS4

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Tags

IRES CHERRY

Expression

Mammalian

Mutation

Wild Type

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-IgL-C99-T4L-rTA

Plasmid

#177840

Purpose

Tango assay

Depositor

Karsten Melcher

Article

Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

Insert

C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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PTEN shRNA

Plasmid

#86645

Purpose

expression of shRNA targeting PTEN

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (PTEN Human)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGRG36-Kn-PA1-GFP

Plasmid

#79088

Purpose

pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

Depositor

Frank Rosenzweig

Article

Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

Insert

GFP

Use

Bacterial chromosome integration and expression o…

Expression

Bacterial

Promoter

PA1

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Nr4a1

Plasmid

#207194

Purpose

doxycycline-inducible expression of Mouse Nr4a1 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NR4A1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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