We narrowed to 7,705 results for: ALP

Showing: 6901 - 6920 of 7705 results

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pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187945

Purpose

dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SpdCas9-tagRFPt-P2A-tagBFP

Use

Synthetic Biology; Piggybac

Tags

NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2-IDR-mCherry-CRY2-NLS

Plasmid

#145267

Purpose

Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2wt-IDR (RUNX2 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLC-RFP657-CASP8

Plasmid

#75164

Purpose

LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

CASP8 sgRNA (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEF1α-mouse-full-length-CHMP3-HA

Plasmid

#154176

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YD EGFP

Plasmid

#187264

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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GST-GINA3

Plasmid

#74979

Purpose

Expresses GST tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus norvegicus G protein subunit alpha i3 (Gnai3) (Gnai3 Rat)

Tags

GST

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAA1 gRNA (BRDN0001146526)

Plasmid

#76253

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF10_P2A_Hygro_Barcode

Plasmid

#120496

Purpose

Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF10 (KLF10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-CEBPa-WT

Plasmid

#215601

Purpose

For overexpression of Gal4-DBD CEBPa WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-GRM3-VC

Plasmid

#98968

Purpose

myc-tagged human mGluR3 fused to C-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Tags

Signal/leader sequence from HLA class I A-2 alpha…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRS-mTagRFP-TUBA1B

Plasmid

#62850

Purpose

Donor vector for gene editing at human TUBA1B locus

Depositor

Gaudenz Danuser

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Insert

mTagRFP-TUBA1B (TUBA1B Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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GPR45-Tango

Plasmid

#66360

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR45 (GPR45 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
EF1a_DIO_R106W-HaloTag

Plasmid

#164063

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 R106W (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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aL-L3

Plasmid

#103881

Purpose

full-length human aL with moxsynGFP in betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with moxsynGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170996

Purpose

HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-APPL1-R146A/K152A/R154A

Plasmid

#59767

Purpose

Expresses APPL1 Endosomal Localization Mutant

Depositor

Donna Webb

Article

Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

Insert

APPL1 (APPL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R146A/K152A/R154A

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_RBM39_VMP1

Plasmid

#205910

Purpose

Express mEGFP-tagged fusion protein, RBM39_VMP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RBM39_VMP1 (RBM39, VMP1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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