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sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

Plasmid

#239608

Purpose

Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

filler-trcr-H1-filler

Use

CRISPR and Lentiviral

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

Plasmid

#46617

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-375 Decoy (MIR375, Mir375 Mouse, Human)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-syn-FLEX-splitTVA-EGFP-tTA

Plasmid

#100798

Purpose

helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

Depositor

Ian Wickersham

Article

Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

Has Service

AAV1

Insert

TVA, EGFP, tet transactivator

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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B*27:02:01

Plasmid

#203269

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*27:02:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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A*32:01:01

Plasmid

#203287

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*32:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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B*13:01:01

Plasmid

#203243

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*13:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Ec-Kbp-D9N

Plasmid

#178011

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Ec-Kbp-D9N

Expression

Bacterial

Promoter

araB

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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OTR untagged

Plasmid

#208913

Purpose

encodes the human oxytocin receptor, without any tags

Depositor

Christian Gruber

Article

Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

Insert

OXTR (OXTR Human)

Expression

Mammalian

Mutation

TM5: 5x56, A218, Threonin instead of Alanin

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244072

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAV CAMKII PSAM4 GlyR IRES EGFP

Plasmid

#119744

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CamKII

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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LAP2 Full I pAcGFP-N1 monomeric GFP (1317)

Plasmid

#62044

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LAP2beta (Tmpo Rat)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pQTEV-LRPAP1

Plasmid

#31327

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

low density lipoprotein receptor-related protein associated (LRPAP1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pIRESpuroN3xFLAG-UFSP2 C302A

Plasmid

#204554

Purpose

Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFSP2 (UFSP2 Human)

Expression

Mammalian

Mutation

C302A

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Expression

Mammalian

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_3xFLAG_V5_ eGFP_V5

Plasmid

#160111

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG, V5

Expression

Mammalian

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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