We narrowed to 6,273 results for: nls
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Plasmid#130816PurposeExpresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cellsDepositorInsertbJun-NFAST-NLS-DEVDG-mCherry-NES
ExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
Plasmid#51082PurposeCre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV1InsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhEF1aAvailable SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA
Plasmid#104563PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)DepositorInsertdLbCpf1 (D832A)
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterCAGAvailable SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST
Plasmid#109048PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vectorDepositorHas ServiceAAV9InserteGtACR1-ST
UseAAVPromoterCAGAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA
Plasmid#108729PurposeExpresses N-terminus to aa270 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Plasmid#62373PurposeExpression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cellsDepositorInsert(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
Tags(-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…ExpressionBacterialMutationY67S amino acid substitution in (-30)GFP renders …PromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
Plasmid#84603PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsODC1 destabilizing elementPromoter8xGliBS-delta-crystallin minimal promoterAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Plasmid#108730PurposeExpresses aa271 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Plasmid#108748PurposeExpresses aa28 to C-terminus of Flp recombinase fused to GAI CIDDepositorInsertFlp recombinase fragment aa28 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA
Plasmid#108723PurposeExpresses N-terminus to aa229 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa229
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA
Plasmid#108724PurposeExpresses aa230 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa230 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS
Plasmid#145268PurposeMammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLSDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS
Plasmid#145270PurposeMammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLSDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS
Plasmid#145269PurposeMammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLSDepositorInsertTBP(38Q)-IDR (TBP Human)
ExpressionMammalianAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
Plasmid#159803PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro beta-catenin P2A eGFP-NLS
Plasmid#155189PurposePiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFPDepositorInsertbeta-catenin (Ctnnb1 Mouse)
UseUnspecified; Piggybac transposonTagsP2A eGFP NLSExpressionMammalianMutationSites phosphorylated by GSK-3beta have been mutat…PromoterTRE3GAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR
Plasmid#136514PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vectorDepositorInsertCodon-optimized AcrIIA5
UseLentiviralTagsFLAG/NLSExpressionMammalianPromoterSFFVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantPromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SARS-CoV2 N P2A eGFP-NLS
Plasmid#154399PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFPDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianPromoterTRE3GAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut
Plasmid#42045DepositorInsertTNRC6A (TNRC6A Human)
TagsFLAG, HA, and SBPExpressionMammalianMutationR930A, R931A, R932A, R934AAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101102PurposeEncodes dCas9(C)-VP64 with SAM components fused to transmembrane tetherDepositorInsertTMt-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsHA and MycExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA
Plasmid#108743PurposeExpresses N-terminus to aa256 of Cre recombinase fused to ABI CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA
Plasmid#108744PurposeExpresses aa257 to C-terminus of Cre recombinase fused to PYL CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA
Plasmid#108789PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108790PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEH280 NLS dTomato 3x high affinity MS2 loops
Plasmid#168211PurposeMammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA
Plasmid#108771PurposeExpresses N-terminus to aa172 of VCre recombinase fused to GID1 CIDDepositorInsertVCre recombinase fragment N-terminus to aa172
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA
Plasmid#108772PurposeExpresses aa173 to C-terminus of VCre recombinase fused to GAI CIDDepositorInsertVCre recombinase fragment aa173 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA
Plasmid#47456PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.DepositorInsertTALE(Grm2)-GS-CIB1(NLS*,∆318-334)
UseAAV; TaleTagsHAExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA
Plasmid#108816PurposeExpresses aa469 to C-terminus of Bxb1 integrase fused to GAI CIDDepositorInsertBxb1 integrase fragment aa469 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA
Plasmid#108797PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA
Plasmid#108795PurposeExpresses N-terminus to aa571 of PhiC31 integrase fused to FRB CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa571
UseCre/Lox and Synthetic BiologyTagsFRB,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA
Plasmid#108796PurposeExpresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CIDDepositorInsertPhiC31 integrase fragment aa572 to C-terminus
UseCre/Lox and Synthetic BiologyTagsFKBP and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA
Plasmid#108805PurposeExpresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa233
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA
Plasmid#108806PurposeExpresses aa234 to C-terminus of PhiC31 integrase fused to GAI CIDDepositorInsertPhiC31 integrase fragment aa234 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#108769PurposeExpresses N-terminus to aa396 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p4949 pLPCX-His-Xpress NLS hBrd4 CTD
Plasmid#14459DepositorInsertBrd4 CTD (BRD4 Human)
UseRetroviralTagsSV40 NLSExpressionMammalianMutationamino acids 1047-1362 of Brd4 (the CTD). Function…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-206-SV40 polyA
Plasmid#190875PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode206
UseAAVMutationWTAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH294 NLS dTomato 1x high affinity MS2 loop
Plasmid#168213PurposeMammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Plasmid#108848PurposeInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMag,NLSExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB003 ZF1/2x6-C 3xFLAG-NLS-VP16-FRB
Plasmid#161546PurposeInducible expression of 3xFLAG-NLS-VP16-FRB under the ZF1/2x6-C promoterDepositorInsert3xFLAG-NLS-VP16-FRB
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterZF1/2x6-CAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
Plasmid#112212PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Plasmid#108846PurposeInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMag,NLSExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only