We narrowed to 26,139 results for: Vit

1. pEGFP ratAPOBEC1 L173A/G227A

   Plasmid

   #167169

   Purpose

   Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

   Depositor

   Silvestro Conticello

   Article

   Chieca et al bioRxiv 2021

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 173 to Alanine; changed Glycine 2…

   Promoter

   CMV promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. attB-P7-attP-YFP

   Plasmid

   #195571

   Purpose

   A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

   Depositor

   Richard Murray

   Article

   Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

   Insert

   Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P7

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRCHisB-TRF2_M

   Plasmid

   #50490

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 Myb (TERF2 Human)
   

   Use

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 438-500

   Promoter

   pTrc

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCheck2 Dest (R1-R2) #BGV129

   Plasmid

   #48955

   Purpose

   Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Luciferase; Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pT7-SpCas9_sgRNA-site2 (RTW448)

   Plasmid

   #160137

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSADdeltaG-GFP-AlstR

   Plasmid

   #32647

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   AlstR (AstA-R1 Fly)
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

7. mCardinal-CytERM-N-17

   Plasmid

   #56158

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   CytERM
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. SaKKH-ABE8e-dimer

   Plasmid

   #138503

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Gdf7

   Plasmid

   #163597

   Purpose

   Expresses GDF-7 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Gdf7 (Gdf7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Some Guanin and Cytosine were substituted to redu…

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL7.0: Venus-oLID-Mito (From ActA)

    Plasmid

    #60414

    Purpose

    Mammalian Expression of Venus-oLID-Mito

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-oLID-Mito
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LbABE8e-dimer

    Plasmid

    #138505

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-miRE_shBRD9_508-SV40-GFP

    Plasmid

    #75138

    Purpose

    Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBRD9_508
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-eGFP-OPTN S513A

    Plasmid

    #175764

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S513A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSETa-Amrose v3

    Plasmid

    #79539

    Purpose

    pRSETa (Invitrogen) plasmid expressing Amrose variant 3

    Depositor

    Randolph Caldwell

    Article

    Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

    Insert

    Amrose v3
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-fSFRP2-GFP

    Plasmid

    #22915

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu secreted frizzled receptor protein 2 promoter driving GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCAGGs-Hoxc8

    Plasmid

    #45555

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc8 (HOXC8 Chicken)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-HA-mIFITM1-C49,50,83,103A

    Plasmid

    #58421

    Purpose

    Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 49, 50, 83, and 103 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

    Plasmid

    #91873

    Purpose

    Photo-activatable/light sensitive Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-E1.1-NRSE-RFP

    Plasmid

    #22929

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    E1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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