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pCW57.1 hPHGDH

Plasmid

#154916

Purpose

Doxycyclin inducible expression of human PHGDH

Depositor

Richard Possemato

Article

Richard et al Richard Possemato Lab plasmids

Insert

PHGDH (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTH385-GST-TEV-SUP35

Plasmid

#29744

Depositor

Tobias von der Haar

Insert

SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR1c-V5/HIS

Plasmid

#201106

Purpose

expression of human FGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-rsChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183523

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

rsChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

I146M/G174S

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PGKp-GFP-YAP

Plasmid

#174172

Purpose

Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EF1a-mEmerald-CAPRIN1

Plasmid

#164218

Purpose

Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

CAPRIN1 (CAPRIN1 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF-1a

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCE-K2N

Plasmid

#154879

Purpose

Episomally expresses KLF2 and NANOG in mammalian cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Insert

KLF2-2A-NANOG (KLF2, NANOG Human)

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCE-KdGl

Plasmid

#154880

Purpose

Episomally expresses KDM4D and GLIS1 in mammalian cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Insert

KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Plasmid

#159807

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-PIK3C2A

Plasmid

#161988

Purpose

Mammalian expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant, Volker Haucke

Article

Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

Insert

PIK3C2A (PIK3C2A Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-NG

Plasmid

#159977

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-NG

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TetOn-CLIMP63-mCherry-eGFP

Plasmid

#139871

Purpose

Dox-inducible lentiviral expression of human CLIMP63

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CLIMP63 (CKAP4 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCrispr-V2-mOrange (V2mO)

Plasmid

#140206

Purpose

Lenti Crispr/Cas9 with mOrange fluorescent marker

Depositor

Jaffer Ajani

Article

Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pELF1a

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ALKBH5H204A-dCas9

Plasmid

#134784

Purpose

Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Mutation

H204A

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-scFv GCN4-sfGFP-VP64

Plasmid

#122133

Purpose

Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

scFv GCN4-sfGFP-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-SV40

Plasmid

#117813

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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