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Showing: 10741 - 10770 of 11014 results

  2. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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  11. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available Since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  12. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  17. pMDC32B-BS-AtMIR390a-B/c

    Plasmid
    #199560
    Purpose
    Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10
    Insert
    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Promoter
    2x35S
    Available Since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    199560-plasmid-map-sequence-id-398170

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  20. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Article
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  21. pMMACE DEST CMV H2B iRFP

    Plasmid
    #206253
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B iRFP713
    Use
    Recombinant baculovirus production, multimate/gat…
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206253-plasmid-map-sequence-id-406403

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  25. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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  27. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid
    #127483
    Purpose
    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    Expression
    Bacterial
    Mutation
    One nucleotide inserted between lambda CI and LGF…
    Available Since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127483-plasmid-map-sequence-id-262924

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  30. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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Showing: 10741 - 10770 of 11014 results